PHF11 Human

PHF11 Protein Human Recombinant
Cat. No.
BT3520
Source
Escherichia Coli.
Synonyms
PHD finger protein 11, BRCA1 C-terminus-associated protein, Renal carcinoma antigen NY-REN-34, PHF11, BCAP, APY, IGEL, IGER, IGHER, NYREN34, NY-REN-34, RP11-185C18.3.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PHF11 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 351 amino acids (1-331 a.a.) and having a molecular mass of 39.7kDa.
PHF11 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
PHD finger protein 11 (PHF11) is a protein that helps control the production of certain signaling molecules in the immune system. Lower PHF11 levels are associated with a specific genetic profile and may contribute to stronger allergic responses. Studies have found connections between PHF11, higher levels of allergy-related antibodies (IgE), asthma, and atopic dermatitis in children. Although PHF11 has a structural component often seen in proteins that regulate gene activity, its exact function remains unclear.
Description
This product is a laboratory-produced version of the human PHF11 protein. It is a single chain of 351 amino acids, with a molecular weight of 39.7kDa. The protein is not glycosylated and has a 20 amino acid His-tag attached to its N-terminus for purification purposes.
Physical Appearance
A clear liquid, free from bacteria or other microorganisms.
Formulation
This solution contains 1mg/ml of PHF11 protein in a buffer solution (pH 8.0) with 20mM Tris-HCl, 0.4M urea, and 10% glycerol.
Stability
For best results, store the product at 4°C and use within 2-4 weeks. For long-term storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeatedly freezing and thawing the product.
Purity
This product contains over 85% pure PHF11 protein, as determined by SDS-PAGE analysis.
Synonyms
PHD finger protein 11, BRCA1 C-terminus-associated protein, Renal carcinoma antigen NY-REN-34, PHF11, BCAP, APY, IGEL, IGER, IGHER, NYREN34, NY-REN-34, RP11-185C18.3.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAQASPPRPE RVLGASSPEA RPAQEALLLP TGVFQVAEKM EKRTCALCPK DVEYNVLYFA QSENIAAHEN CLLYSSGLVE CEDQDPLNPD RSFDVESVKK EIQRGRKLKC KFCHKRGATV GCDLKNCNKN YHFFCAKKDD AVPQSDGVRG IYKLLCQQHA QFPIIAQSAK FSGVKRKRGR KKPLSGNHVQ PPETMKCNTF IRQVKEEHGR HTDATVKVPF LKKCKEAGLL NYLLEEILDK VHSIPEKLMD ETTSESDYEE IGSALFDCRL FEDTFVNFQA AIEKKIHASQ QRWQQLKEEI ELLQDLKQTL CSFQENRDLM SSSTSISSLS Y.

Product Science Overview

Introduction

The PHF11 protein, also known as Plant Homeodomain Finger Protein 11, is a zinc finger protein encoded by the PHF11 gene. This protein is involved in various cellular processes, including immune response and gene regulation. The recombinant form of this protein is produced through genetic engineering techniques, allowing for its use in research and therapeutic applications.

Gene and Protein Structure

The PHF11 gene is located on chromosome 13 and encodes a protein containing a PHD (plant homeodomain) type zinc finger . This domain is known for its role in mediating protein-protein interactions and binding to DNA, which is crucial for the regulation of gene expression.

Expression and Localization

PHF11 is highly expressed in circulating immune cells, particularly in T-helper 1 (Th1) cells compared to T-helper 2 (Th2) cells . It is also expressed in various tissues, including lymphoid tissue, bone marrow, testis, and skeletal muscle . The protein’s expression and cellular distribution are regulated by the Toll-Like Receptor 3 Ligand Polyinosinic:Polycytidylic Acid in keratinocytes .

Function and Role in Immune Response

PHF11 plays a significant role in the immune system. It regulates the transcription of T-cell cytokines and class switching to IgE in activated B-cells . Knock-down of PHF11 using small interfering RNA (siRNA) decreases the expression of the interferon-γ (IFNG) gene in Th1 cells through a mechanism involving a reduction in NFκB-dependent transcriptional activity . This protein is also involved in the innate immune response of keratinocytes, contributing to epidermal recovery following infection or other damage .

Clinical Significance

PHF11 has been identified as a candidate gene for asthma and other allergic diseases . Polymorphisms in this gene have been associated with dermatitis and allergy . The protein’s role in regulating immune responses makes it a potential target for therapeutic interventions in inflammatory and allergic conditions.

Research and Applications

Recombinant PHF11 protein is used in various research applications to study its function and role in disease. It is also utilized in drug development and therapeutic research, particularly in the context of immune-related disorders.

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