PGP9.5 Antibody

PGP9.5, Mouse Anti Human
Cat. No.
BT29676
Source
Synonyms
Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1, EC 3.4.19.12, Ubiquitin thioesterase L1, Neuron cytoplasmic protein 9.5, PGP 9.5, UCHL1, PGP9.5, PARK5.
Appearance
Sterile Filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
UCHL1 (PGP9.5) is a member of a gene family whose products function to hydrolyze small C-terminal adducts of ubiquitin, resulting in the production of ubiquitin monomers. Protein Gene Product (PGP9.5) is a neuron-specific protein that is structurally and immunologically distinct from neuron-specific enolase. The human forms of UCHL1 and UCHL3 possess a remarkably complex knot structure, characterized by five knot crossings, which is unusual for proteins. It is hypothesized that this intricate knot structure might enhance the protein's resistance to degradation by the proteasome. With a molecular weight of 27 kDa, the protein was initially identified through high-resolution two-dimensional PAGE. Standard immunohistochemical techniques have revealed the presence of PGP9.5 in various locations, including neurons and nerve fibers throughout the central and peripheral nervous systems, numerous neuroendocrine cells, specific segments of renal tubules, spermatogonia and Leydig cells in the testis, ova, and certain cells within both the pregnant and non-pregnant corpus luteum. Notably, a point mutation (I93M) in UCHL1 has been implicated in disease.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The antibody is supplied at a concentration of 1 mg/ml in a solution of PBS at pH 7.4 with 0.1% sodium azide.
Storage Procedures
For short-term storage (up to 1 month), the antibody can be stored at 4°C. For longer storage durations, it is recommended to store the antibody at -20°C. Repeated freezing and thawing of the antibody should be avoided.
Stability / Shelf Life
The antibody has a shelf life of 12 months when stored at -20°C. When stored at 4°C, the antibody is stable for 1 month.
Applications
The PGP9.5 antibody has undergone rigorous testing in ELISA, Western blot, and immunohistochemistry analyses to ensure its specificity and reactivity. However, it's important to note that optimal working dilutions may vary depending on the specific application and should be determined through titration. For Western blot analysis, a dilution range of 1:500 to 1:2,000 is recommended, with a starting dilution of 1:1,000. For immunohistochemistry analysis, a dilution range of 1:100 to 1:200 is suggested, with a starting dilution of 1:100.
Synonyms
Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1, EC 3.4.19.12, Ubiquitin thioesterase L1, Neuron cytoplasmic protein 9.5, PGP 9.5, UCHL1, PGP9.5, PARK5.
Purification Method
PGP9.5 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
P3D9AT.
Immunogen
Anti-human PGP9.5 mAb is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PGP9.5 amino acids 1-223 purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and κ light chain.

Product Science Overview

Introduction

Protein Gene Product 9.5 (PGP9.5), also known as ubiquitin C-terminal hydrolase 1 (UCHL-1), is a protein that plays a crucial role in the ubiquitin-proteasome system. This system is essential for the degradation of misfolded or damaged proteins, thereby maintaining cellular homeostasis. PGP9.5 is widely expressed in neuronal tissues and represents a significant portion of the total soluble brain proteins .

Biological Function

PGP9.5 is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin . This action is vital for the recycling of ubiquitin, a small regulatory protein that tags other proteins for degradation. By recycling ubiquitin, PGP9.5 helps regulate protein turnover and prevents the accumulation of damaged or misfolded proteins, which could otherwise lead to cellular dysfunction and disease .

Expression and Localization

PGP9.5 is predominantly expressed in neuronal tissues, including the central and peripheral nervous systems . It is found in neuronal cell bodies and axons, as well as in small nerve fibers in peripheral tissues . Additionally, PGP9.5 is present in neuroendocrine cells in the pituitary, thyroid, and pancreas, as well as in tumors of the diffuse neuroendocrine system .

Mouse Anti Human PGP9.5 Antibody

The mouse anti human PGP9.5 antibody, particularly clone 31A3, is a monoclonal antibody that specifically recognizes PGP9.5 . This antibody has been widely used in research to study the expression and localization of PGP9.5 in various tissues. It stains neuronal cell bodies and axons in the central and peripheral nervous systems, as well as small nerve fibers in peripheral tissues . The antibody has also been used to detect PGP9.5 in neuroendocrine cells and tumors .

Applications

The mouse anti human PGP9.5 antibody has several applications in research. It has been used in immunohistochemistry to study the distribution of PGP9.5 in tissues . Additionally, it has been employed in western blotting to detect PGP9.5 protein levels in various samples . The antibody has also been utilized in enzyme-linked immunosorbent assays (ELISAs) to evaluate the presence of neuronal tissue contamination in processed meat samples .

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