PGM2 Human

PGM2 is a protein coding gene that encodes a single, non-glycosylated polypeptide chain containing 635 amino acids, with a molecular mass of approximately 70.7 kDa . The enzyme catalyzes the conversion of nucleoside breakdown products, such as ribose-1-phosphate and deoxyribose-1-phosphate, to their corresponding 5-phosphopentoses . Additionally, PGM2 facilitates the reversible isomerization of alpha-D-glucose 1-phosphate to alpha-D-glucose 6-phosphate through an intermediate compound, alpha-D-glucose 1,6-bisphosphate .

PGM2 is involved in several metabolic processes, including the carbohydrate metabolic process and the deoxyribose phosphate catabolic process . It is predicted to act upstream of or within the glucose metabolic process and is located in extracellular exosomes . The enzyme’s activity is crucial for maintaining proper glucose levels and ensuring efficient energy production within cells.

Mutations or deficiencies in the PGM2 gene can lead to various disorders. For instance, diseases associated with PGM2 include Congenital Disorder of Glycosylation, Type In, and Cerebellar Ataxia Type 47 . These conditions highlight the importance of PGM2 in normal cellular function and development.

Human recombinant PGM2 is produced in Escherichia coli and is used for various biological assays . The recombinant protein is typically formulated as a sterile, filtered, colorless solution containing phosphate-buffered saline and glycerol for stability . It is essential for research purposes, particularly in studies related to carbohydrate metabolism and related disorders.

Recombinant PGM2 should be stored at 4°C if it will be used within 2-4 weeks. For longer periods, it should be stored frozen at -20°C. To ensure long-term stability, it is recommended to add a carrier protein, such as human serum albumin or bovine serum albumin, and avoid multiple freeze-thaw cycles .