PGM1 Antibody

Phosphoglucomutase 1, Mouse Anti Human
Cat. No.
BT22597
Source
Synonyms
PGM1, Phosphoglucomutase 1, Glucose Phosphomutase 1, EC 5.4.2.2, PGM 1, CDG1T, GSD14, Phosphoglucomutase-1, EC 5.4.2.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

Product Specs

Introduction
Phosphoglucomutase-1, also referred to as PGM1, belongs to the phosphohexose mutase family. Several PGM isozymes exist, facilitating the transfer of phosphate between the 1 and 6 positions of glucose. PGM1 isozymes are the predominant form in most cell types, accounting for approximately 90% of total PGM activity. Notably, defects in PGM1 have been identified as the underlying cause of glycogen storage disease type 14.
Physical Appearance
The product appears as a clear, colorless solution that has been sterilized by filtration.
Formulation

The antibody solution has a concentration of 1mg/ml and is prepared in a buffer containing PBS at pH 7.4, 10% Glycerol, and 0.02% Sodium Azide.

Storage Procedures
For short-term storage of up to one month, the product should be kept at a temperature of 4°C. For extended storage periods, it is recommended to store the product at -20°C. Repeated freezing and thawing of the product should be avoided.
Stability / Shelf Life
The product remains stable for a period of 12 months when stored at -20°C. Alternatively, it can be stored at 4°C for up to 1 month.
Applications
This antibody has undergone rigorous testing in various applications, including ELISA, Western blot analysis, Flow cytometry, and ICC/IF, to confirm its specificity and reactivity. However, it's essential to note that optimal working dilutions may vary depending on the specific application. Therefore, users are advised to perform a titration to determine the most effective concentration for their experimental needs.
Synonyms
PGM1, Phosphoglucomutase 1, Glucose Phosphomutase 1, EC 5.4.2.2, PGM 1, CDG1T, GSD14, Phosphoglucomutase-1, EC 5.4.2.
Purification Method
PGM1 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT84G2AT.
Immunogen
Anti-human PGM1 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PGM1 protein 1-562 amino acids purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and k light chain.

Product Science Overview

Mouse Anti-Human Antibodies

Mouse anti-human antibodies are monoclonal antibodies produced by immunizing mice with human antigens. These antibodies are widely used in research and clinical diagnostics due to their high specificity and affinity for human proteins .

Production and Applications

Mouse anti-human antibodies are generated by immunizing mice with human proteins or peptides. The immune response in mice leads to the production of antibodies that specifically recognize and bind to the human antigen. These antibodies can then be harvested and purified for use in various applications, including:

  • Western Blotting: To detect specific proteins in a sample.
  • ELISA: For quantifying the presence of antigens or antibodies in a sample.
  • Flow Cytometry: To analyze the expression of cell surface and intracellular molecules.
  • Immunohistochemistry: For visualizing the distribution and localization of proteins within tissue sections .

Specificity and Cross-Reactivity

Mouse anti-human antibodies are designed to have high specificity for human proteins, minimizing cross-reactivity with proteins from other species. This specificity is achieved through careful selection and screening of the antibodies during the production process .

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