Introduction
Phosphoglucomutase-1, also referred to as PGM1, belongs to the phosphohexose mutase family. Several PGM isozymes exist, facilitating the transfer of phosphate between the 1 and 6 positions of glucose. PGM1 isozymes are the predominant form in most cell types, accounting for approximately 90% of total PGM activity. Notably, defects in PGM1 have been identified as the underlying cause of glycogen storage disease type 14.
Physical Appearance
The product appears as a clear, colorless solution that has been sterilized by filtration.
Formulation
The antibody solution has a concentration of 1mg/ml and is prepared in a buffer containing PBS at pH 7.4, 10% Glycerol, and 0.02% Sodium Azide.
Storage Procedures
For short-term storage of up to one month, the product should be kept at a temperature of 4°C. For extended storage periods, it is recommended to store the product at -20°C. Repeated freezing and thawing of the product should be avoided.
Stability / Shelf Life
The product remains stable for a period of 12 months when stored at -20°C. Alternatively, it can be stored at 4°C for up to 1 month.
Applications
This antibody has undergone rigorous testing in various applications, including ELISA, Western blot analysis, Flow cytometry, and ICC/IF, to confirm its specificity and reactivity. However, it's essential to note that optimal working dilutions may vary depending on the specific application. Therefore, users are advised to perform a titration to determine the most effective concentration for their experimental needs.
Synonyms
PGM1, Phosphoglucomutase 1, Glucose Phosphomutase 1, EC
5.4.2.2, PGM 1, CDG1T, GSD14, Phosphoglucomutase-1, EC 5.4.2.
Purification Method
PGM1 antibody was purified from mouse ascitic fluids by
protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Immunogen
Anti-human PGM1 mAb, is
derived from hybridization of mouse F0 myeloma cells with spleen cells from
BALB/c mice immunized with a recombinant human PGM1 protein 1-562 amino
acids purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and k light chain.