PGLYRP1 Human, HEK
HEK293 Cells.
PGLYRP, PGRP, PGRP-S, PGRPS, TAG7, TNFSF3L, Peptidoglycan recognition protein 1, peptidoglycan recognition protein short.
Sterile filtered colorless solution.
Greater than 95.0% as determined by SDS-PAGE.
Description
PGLYRP1 Human Recombinant produced in HEK293 Cells is a single, glycosylated polypeptide chain containing 184 amino acids (22-196 a.a) and having a molecular mass of 20.5 kDa.
PGLYRP1 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Peptidoglycan Recognition Protein 1, also known as PGLYRP1, is a protein that plays a crucial role in the innate immune system's defense against bacteria. It specifically targets peptidoglycan, a vital component of bacterial cell walls. PGLYRP1 exhibits bactericidal activity against Gram-positive bacteria, effectively killing them, while it demonstrates bacteriostatic activity against Gram-negative bacteria, inhibiting their growth. This protein is primarily produced in the bone marrow but can also be found in lower concentrations in other organs such as the kidney, liver, and small intestine. Additionally, PGLYRP1 is present in immune cells like neutrophils and lymphocytes.
This product consists of the recombinant human PGLYRP1 protein, produced in HEK293 cells. It is a single polypeptide chain with a molecular weight of 20.5 kDa. The protein sequence spans from amino acid residues 22 to 196 and includes a 6-amino acid His tag located at the C-terminus to facilitate purification. The protein undergoes glycosylation, a common post-translational modification, and is purified using proprietary chromatographic techniques to ensure high purity and activity.
The product appears as a clear, colorless solution that has been sterilized by filtration.
This PGLYRP1 solution is provided at a concentration of 0.25 mg/ml and is formulated in a phosphate-buffered saline (PBS) solution with a pH of 7.4. To enhance stability, the solution also contains 10% glycerol.
To maintain product integrity, it is recommended to store the vial at 4°C if the entire volume will be used within 2-4 weeks. For extended storage, the solution should be frozen at -20°C. The addition of a carrier protein, such as 0.1% HSA or BSA, is advisable for long-term storage to further enhance stability. It is crucial to avoid repeated cycles of freezing and thawing to prevent protein degradation and loss of activity.
The purity of this product is greater than 95%, as determined by SDS-PAGE analysis, a widely used technique for assessing protein purity.
The biological activity of this PGLYRP1 protein was assessed using a functional ELISA, which measures its ability to bind to peptidoglycan. The ED50 value, representing the concentration at which 50% binding is observed, is less than or equal to 30 ng/ml, indicating its high affinity for peptidoglycan.
PGLYRP, PGRP, PGRP-S, PGRPS, TAG7, TNFSF3L, Peptidoglycan recognition protein 1, peptidoglycan recognition protein short.
HEK293 Cells.
DGSQETEDPA CCSPIVPRNE WKALASECAQ HLSLPLRYVV VSHTAGSSCN TPASCQQQAR NVQHYHMKTL GWCDVGYNFL IGEDGLVYEG RGWNFTGAHS GHLWNPMSIG ISFMGNYMDR VPTPQAIRAA QGLLACGVAQ GALRSNYVLK GHRDVQRTLS PGNQLYHLIQ NWPHYRSPHH HHHH
Product Science Overview
PGLYRP1 was independently discovered by two laboratories in 1998. Håkan Steiner and his team identified and cloned Peptidoglycan Recognition Protein (PGRP) in a moth and subsequently discovered mouse and human orthologs . Sergei Kiselev and his colleagues also cloned a protein from a mouse adenocarcinoma with the same sequence as mouse PGRP, naming it Tag7 .
The PGLYRP1 gene is located on chromosome 19 in humans . The protein is characterized by its ability to bind to peptidoglycan, a major component of bacterial cell walls, and is involved in the immune response to bacterial infections .
PGLYRP1 is primarily involved in the innate immune response. It binds to peptidoglycan and exhibits antibacterial activity by recognizing and binding to bacterial cell wall components . This binding triggers a series of immune responses, including the activation of immune cells and the production of pro-inflammatory cytokines .
The protein is expressed in various tissues, including the bone marrow, blood granulocytes, spleen, and liver . It plays a significant role in the defense against Gram-positive bacteria and is involved in processes such as neutrophil degranulation and the antimicrobial humoral response .
PGLYRP1 has been studied for its role in various diseases and conditions. For instance, it has been found to confer immune evasive properties on pancreatic cancer stem cells, highlighting its potential role in cancer biology . Additionally, its involvement in the immune response makes it a target for research in infectious diseases and inflammatory conditions.
