PGD Antibody
Description
Product Specs
Product Science Overview
Mouse anti-human antibodies are secondary antibodies produced by immunizing mice with human immunoglobulins. These antibodies are used in various immunological assays to detect, sort, or purify human proteins. They are affinity-purified to ensure high specificity and are often conjugated with labels such as HRP, AP, or fluorescence for detection purposes .
Applications
Mouse anti-human antibodies are widely used in:
- ELISA (Enzyme-Linked Immunosorbent Assay): For detecting and quantifying specific proteins.
- Western Blotting: For identifying proteins in a sample.
- Flow Cytometry: For sorting and analyzing cells.
- Immunohistochemistry: For visualizing proteins in tissue sections .
HAMA Response
One potential issue with using mouse anti-human antibodies is the development of the Human Anti-Mouse Antibody (HAMA) response. This is an allergic reaction that can range from mild (e.g., rash) to severe (e.g., kidney failure) . Researchers often use various strategies to minimize this response, such as using humanized antibodies or pre-adsorbing the antibodies to remove cross-reactive components.
