PFN1 Rat
Description
Product Specs
Product Science Overview
Profilin-1 is a ubiquitous actin-binding protein that plays a crucial role in the regulation of the cytoskeleton. It is involved in various cellular processes, including cell motility, division, and signaling. Profilin-1 is highly conserved across species, and its recombinant form from rats is widely used in research to study its functions and interactions.
Recombinant Rat Profilin-1 is a full-length protein consisting of 140 amino acids. It is typically expressed in Escherichia coli and purified to a high degree of purity (>95%) using conventional chromatography techniques . The protein is often tagged with a His-tag at the N-terminus to facilitate purification and detection .
Profilin-1 binds to actin monomers (G-actin) and regulates actin polymerization. At high concentrations, profilin-1 prevents the polymerization of actin, while at low concentrations, it enhances actin polymerization . This dual role is essential for maintaining the dynamic nature of the cytoskeleton.
Profilin-1 also interacts with phosphatidylinositol 4,5-bisphosphate (PIP2), inhibiting the formation of inositol trisphosphate (IP3) and diacylglycerol (DG). This interaction plays a role in various signaling pathways .
Profilin-1 has been implicated in several pathological conditions. For instance, its levels are increased in the vascular endothelium under pathological conditions such as diabetes . Overexpression of profilin-1 has been shown to trigger indicators of endothelial dysfunction downstream of low-density lipoprotein (LDL) signaling . Additionally, profilin-1 expression is significantly enhanced in human atherosclerotic plaques compared to normal vessel walls .
Recombinant Rat Profilin-1 is widely used in research to study its role in actin dynamics and its interactions with other proteins. It is suitable for various applications, including SDS-PAGE and mass spectrometry (MS) . Researchers use recombinant profilin-1 to investigate its effects on cellular processes such as DNA synthesis, chemotaxis, and intracellular signaling pathways .
