PFDN6 Human
Description
PFDN6 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Prefoldin Subunit 6 (PFDN6) is a crucial component of the prefoldin complex, a molecular chaperone that assists in the proper folding of newly synthesized proteins. This article delves into the background, structure, function, and significance of Prefoldin Subunit 6, particularly in its recombinant human form.
The prefoldin complex was first discovered in eukaryotes and archaea, where it was identified as a facilitator of cytoskeletal protein assembly, specifically actin and tubulin . The complex is also known as the Gim complex (Genes involved in microtubule biogenesis). Prefoldin plays a vital role in preventing protein aggregation by providing a linear substrate for class II chaperones, such as Hsp60 .
Prefoldin is a heterohexameric complex composed of six subunits: two alpha and four beta subunits . Prefoldin Subunit 6 (PFDN6) is one of the beta subunits. The complex forms a double beta-barrel structure with six protruding coiled-coils . In eukaryotes, there are multiple paralogous genes encoding each subunit, adding to the heterogeneity of the hexamer .
Prefoldin Subunit 6, like other subunits, binds and stabilizes newly synthesized polypeptides, allowing them to fold correctly . This process is crucial for maintaining cellular homeostasis and preventing diseases caused by protein misfolding. PFDN6 has been shown to interact with other subunits in the prefoldin complex, protecting them from degradation mediated by the ubiquitin-proteasome system .
Prefoldin and its subunits, including PFDN6, have been implicated in various diseases, particularly neurodegenerative disorders such as Alzheimer’s, Parkinson’s, and Huntington’s diseases . Abnormal expression of prefoldin subunits has also been observed in different tumors, highlighting their role in tumorigenesis and development .
Recombinant human PFDN6 is produced using recombinant DNA technology, which involves inserting the human PFDN6 gene into a suitable expression system, such as bacteria or yeast. This allows for the large-scale production of the protein, which can be used in research and therapeutic applications.
