PET117 Human

PET117 is located on chromosome V near the HIS1 gene in yeast. The human recombinant version of PET117 is expressed in Escherichia coli and is typically fused with a His-tag at the N-terminus for purification purposes. The recombinant protein corresponds to the amino acids 23-81 of the human PET117 sequence .

The amino acid sequence of the recombinant human PET117 protein is as follows:

MGSSHHHHHH SSGLVPRGSH MGSVHVKQQW DQQRLRDGVI RDIERQIRKK ENIRLLGEQI ILTEQLEAER EKMLLAKGSQ KS

The theoretical molecular weight of this protein is approximately 9.5 kDa, although the observed molecular weight may vary due to post-translational modifications and other experimental factors .

The recombinant human PET117 protein is expressed in Escherichia coli and purified using conventional chromatography techniques. The purity of the protein is greater than 90%, as determined by SDS-PAGE . The protein is typically stored in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.2 M NaCl, 50% glycerol, and 2 mM DTT to maintain its stability .

In yeast, PET117 is required for the assembly of active cytochrome c oxidase, an essential component of the mitochondrial electron transport chain. This enzyme complex is responsible for the final step in the mitochondrial respiratory chain, where electrons are transferred to oxygen, resulting in the formation of water. The proper assembly and function of cytochrome c oxidase are critical for cellular respiration and energy production .

Recombinant human PET117 protein is primarily used in research settings to study its role in mitochondrial function and its potential implications in human health and disease. The protein can be utilized in various applications, including SDS-PAGE and mass spectrometry (MS), to investigate its structure, function, and interactions with other proteins .