Perth 16/09

Hemagglutinin-Influenza A Virus H3N2 Perth 16/09 Recombinant
Cat. No.
BT28171
Source
Baculovirus
Synonyms
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

H3N2 produced in Hi-5 cell of Baculovirus is a single polypeptide chain containing 339 amino acids (17-345) and having a molecular mass of 37.8kDa.
H3N2 is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
H3N2, a subtype of influenza A virus, is named after its surface proteins hemagglutinin (H) and neuraminidase (N). This subtype exchanges internal protein genes with other influenza subtypes and has often been more prevalent than H1N1, H1N2, and influenza B. Originating from H2N2 through antigenic shift, where genes from different subtypes recombined, H3N2, like H2N2, carries genes from avian influenza viruses. This virus infects mammals and birds, mutating into numerous strains within pigs, humans, and birds. Hemagglutinin (HA) facilitates viral attachment and entry by binding to sialic acid receptors on cell surfaces. HA plays a crucial role in determining the virus's host range, virulence, and its ability to fuse with the host cell membrane for cytoplasmic entry.
Description
Produced in Hi-5 insect cells using the Baculovirus expression system, this recombinant H3N2 protein is a single polypeptide chain consisting of 339 amino acids (residues 17-345). It has a molecular weight of 37.8kDa, including a C-terminal 6-His tag for purification. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The H3N2 protein is supplied in a solution at a concentration of 0.5mg/ml. The solution contains 20mM Tris-HCl buffer at pH 8.0 and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the solution can be stored at 4°C. For long-term storage, it is recommended to store the protein frozen at -20°C. Adding a carrier protein such as 0.1% HSA or BSA is advisable for long-term storage. Avoid repeated freezing and thawing of the protein solution.
Purity
The purity of the H3N2 protein is greater than 90% as determined by SDS-PAGE analysis.
Source
Baculovirus
Amino Acid Sequence
ADPMQKLPGN DNSTATLCLG HHAVPNGTIV KTITNDQIEV TNATELVQSS STGEICDSPH QILDGKNCTL IDALLGDPQC DGFQNKKWDL FVERSKAYSN CYPYDVPDYA SLRSLVASSG TLEFNNESFN WTGVTQNGTS SACIRRSKNS FFSRLNWLTH LNFKYPALNV TMPNNEQFDK LYIWGVHHPG TDKDQIFLYA QASGRITVST KRSQQTVSPN IGSRPRVRNI PSRISIYWTI VKPGDILLIN STGNLIAPRG YFKIRSGKSS IMRSDAPIGK CNSECITPNG SIPNDKPFQN VNRITYGACP RYVKQNTLKL ATGMRNVPEK QTRHHHHHH.

Product Science Overview

Introduction

Hemagglutinin (HA) is a glycoprotein found on the surface of the influenza viruses. It plays a crucial role in the virus’s ability to infect host cells. The HA protein is responsible for binding the virus to the cell that is being infected. Influenza A virus H3N2 is one of the subtypes of the influenza virus that has been a significant cause of seasonal flu outbreaks in humans.

Hemagglutinin Structure and Function

The HA protein is composed of two subunits, HA1 and HA2, which are derived from a single polypeptide precursor, HA0. The HA1 subunit contains the receptor-binding site, while the HA2 subunit is involved in the fusion of the viral and cellular membranes. The HA protein undergoes antigenic drift, which is the gradual accumulation of mutations in the HA gene, leading to changes in the antigenic properties of the virus. This antigenic drift is a major reason why influenza vaccines need to be updated regularly.

Influenza A Virus H3N2

The H3N2 subtype of the influenza A virus first emerged in humans in 1968, causing a pandemic known as the “Hong Kong flu.” This subtype has continued to circulate in the human population, causing seasonal flu outbreaks. The H3N2 virus is known for its rapid antigenic drift, which makes it challenging to develop effective vaccines.

Perth 16/09 Strain

The Perth 16/09 strain of the H3N2 virus was identified in 2009. This strain has been included in seasonal influenza vaccines due to its prevalence and antigenic properties. The recombinant form of the HA protein from this strain is used in research and vaccine development to study immune responses and to develop more effective vaccines.

Recombinant Hemagglutinin

Recombinant HA proteins are produced using genetic engineering techniques. These proteins are used in various applications, including vaccine development and serological assays. The recombinant HA from the Perth 16/09 strain is particularly valuable for studying the immune response to the H3N2 virus and for developing vaccines that can provide broader protection against different strains of the virus.

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