PELI2 Human

Pellino E3 Ubiquitin Protein Ligase Family Member 2 Human Recombinant

Cat. No.

BT6479

Source

Escherichia Coli.

Synonyms

Pellino E3 Ubiquitin Protein Ligase Family Member 2, Pellino-2, E3 Ubiquitin-Protein Ligase Pellino Homolog 2, Pellino (Drosophila) Homolog 2, Pellino Homolog 2 (Drosophila), Protein Pellino Homolog 2, Pellino Homolog 2, EC 6.3.2, PELI2.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 80.0% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

PELI2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 443 amino acids (1-420 a.a) and having a molecular mass of 48.8kDa.

PELI2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

Pellino E3 Ubiquitin Protein Ligase Family Member 2, also known as PELI2, is an E3 ubiquitin ligase that facilitates the attachment of ubiquitin molecules to specific substrate proteins. This protein plays a crucial role in the TLR and IL-1 signaling pathways by interacting with a complex containing IRAK kinases and TRAF6. PELI2 is involved in IL1B-induced IRAK1 'Lys-63'-linked polyubiquitination and potentially 'Lys-48'-linked ubiquitination. It is essential for LPS- and IL1B-induced activation of NF-kappa-B, which is dependent on MAP3K7 but not MAP3K3. Additionally, PELI2 can activate the mitogen-activated protein (MAP) kinase pathway, leading to the activation of ELK1.

Description

Recombinant Human PELI2, expressed in E. coli, is a single, non-glycosylated polypeptide chain. This protein consists of 443 amino acids (with amino acids 1-420 being present) and has a molecular weight of 48.8 kDa. The N-terminus of the protein is fused with a 23 amino acid His-tag. Purification is carried out using proprietary chromatographic techniques.

Physical Appearance

A clear, colorless solution that has been sterilized by filtration.

Formulation

The PELI2 protein solution has a concentration of 0.5 mg/ml and is supplied in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, 20% glycerol, and 1 mM DTT.

Stability

For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. To ensure long-term stability, adding a carrier protein (0.1% HSA or BSA) is advisable. Repeated freezing and thawing should be avoided.

Purity

The purity of the protein is determined to be greater than 80.0% using SDS-PAGE analysis.

Synonyms

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMFSPGQE EHCAPNKEPV KYGELVVLGY NGALPNGDRG RRKSRFALYK RPKANGVKPS TVHVISTPQA SKAISCKGQH SISYTLSRNQ TVVVEYTHDK DTDMFQVGRS TESPIDFVVT DTISGSQNTD EAQITQSTIS RFACRIVCDR NEPYTARIFA AGFDSSKNIF LGEKAAKWKN PDGHMDGLTT NGVLVMHPRG GFTEESQPGV WREISVCGDV YTLRETRSAQ QRGKLVESET NVLQDGSLID LCGATLLWRT ADGLFHTPTQ KHIEALRQEI NAARPQCPVG LNTLAFPSIN RKEVVEEKQP WAYLSCGHVH GYHNWGHRSD TEANERECPM CRTVGPYVPL WLGCEAGFYV DAGPPTHAFT PCGHVCSEKS AKYWSQIPLP HGTHAFHAAC PFCATQLVGE QNCIKLIFQG PID.

Product Science Overview

Introduction

Pellino E3 Ubiquitin Protein Ligase Family Member 2 (PELI2) is a protein encoded by the PELI2 gene in humans. This protein is part of the Pellino family of E3 ubiquitin ligases, which play a crucial role in the regulation of immune responses through the ubiquitylation of specific substrate proteins .

Structure and Function

PELI2 is characterized by an N-terminal forkhead-associated (FHA) domain and a C-terminal RING-like domain. The FHA domain recognizes phospho-threonine residues, facilitating the association with interleukin-1 receptor-associated kinases (IRAKs), while the RING-like domain confers E3 ubiquitin ligase activity. This activity involves catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins, a process essential for various cellular functions, including protein degradation, signal transduction, and DNA repair.

Role in Immune Signaling

PELI2 is implicated in the Toll-like receptor (TLR) and interleukin-1 (IL-1) signaling pathways. It interacts with complexes containing IRAK kinases and TNF receptor-associated factor 6 (TRAF6), mediating the polyubiquitination of IRAK1. This modification is crucial for the activation of downstream signaling pathways, including the mitogen-activated protein kinase (MAPK) pathway and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway . These pathways are vital for the innate immune response, leading to the production of pro-inflammatory cytokines and other immune mediators.

Biological Significance

PELI2’s role in immune signaling highlights its importance in both innate and adaptive immunity. By regulating the ubiquitylation of key signaling proteins, PELI2 ensures the proper activation and termination of immune responses. Dysregulation of PELI2 activity has been associated with various immune-related disorders, including hyperlipoproteinemia type IV and 3MC syndrome.

Research and Therapeutic Potential

Given its central role in immune signaling, PELI2 is a potential target for therapeutic interventions aimed at modulating immune responses. Research into the molecular mechanisms of PELI2 function and regulation continues to provide insights into its potential as a therapeutic target for treating immune-related diseases.

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PELI2 Human

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