PEF1 Human

Penta-EF-Hand Domain Containing 1 Human Recombinant
Cat. No.
BT6401
Source
Escherichia Coli.
Synonyms
Peflin, PEF protein with a long N-terminal hydrophobic domain, Penta-EF hand domain-containing protein 1, PEF1, ABP32, PEF1A.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PEF1 Human Recombinant fused with a 36 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 320 amino acids (1-284 a.a.) and having a molecular mass of 34.5kDa. The PEF1 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Penta-EF-hand domain containing 1 (PEF1), a member of the penta-EF-hand protein family, shares similarities with the apoptosis-linked gene ALG-2. It forms heterodimers with PDCD6/ALG2, dissociating in the presence of Ca(2+). PEF1 proteins bind calcium and are involved in various calcium-dependent processes in vertebrates.
Description
Recombinant Human PEF1, fused to a 36 amino acid His tag at its N-terminus, is produced in E. coli. This non-glycosylated polypeptide consists of 320 amino acids (1-284 a.a.) with a molecular mass of 34.5 kDa. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
A sterile, colorless solution.
Formulation
The PEF1 solution (0.25 mg/ml) is formulated in 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 50% glycerol, and 0.2M NaCl.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 85.0% as determined by SDS-PAGE analysis.
Synonyms
Peflin, PEF protein with a long N-terminal hydrophobic domain, Penta-EF hand domain-containing protein 1, PEF1, ABP32, PEF1A.
Source
Escherichia Coli.
Amino Acid Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMASY PYRQGCPGAA GQAPGAPPGS
YYPGPPNSGG QYGSGLPPGG GYGGPAPGGP YGPPAGGGPY GHPNPGMFPS GTPGGPYGGA
APGGPYGQPP PSSYGAQQPG LYGQGGAPPN VDPEAYSWFQ SVDSDHSGYI SMKELKQALV
NCNWSSFNDE TCLMMINMFD KTKSGRIDVY GFSALWKFIQ QWKNLFQQYD RDRSGSISYT
ELQQALSQMG YNLSPQFTQL LVSRYCPRSA NPAMQLDRFI QVCTQLQVLT EAFREKDTAV
QGNIRLSFED FVTMTASRML.

Product Science Overview

Introduction

Penta-EF-Hand Domain Containing 1 (PEF1) is a protein encoded by the PEF1 gene in humans. This protein is part of the penta-EF-hand (PEF) family, which includes several calcium-binding proteins characterized by the presence of five EF-hand motifs. These motifs are helix-loop-helix structures that bind calcium ions, playing crucial roles in various cellular processes.

Structure and Function

The EF-hand motif is a well-known calcium-binding domain found in a large family of proteins. The motif consists of two alpha helices linked by a loop region that binds calcium ions. The PEF1 protein contains five such EF-hand motifs, which allow it to interact with calcium ions and other proteins in a calcium-dependent manner .

PEF1 is involved in several cellular processes, including signal transduction, membrane repair, and vesicular transport. It interacts with various proteins, including those in the endosomal sorting complex required for transport (ESCRT) system . This interaction is crucial for processes such as cell death, intracellular signaling, and RNA processing.

Biological Significance

PEF1 and other PEF family proteins, such as ALG-2 (PDCD6), play significant roles in cellular homeostasis and response to stress. ALG-2, a prototypic PEF protein, is widely distributed in eukaryotes and interacts with a variety of proteins in a calcium-dependent manner . These interactions are essential for maintaining cellular functions and responding to changes in calcium levels.

Clinical Relevance

Mutations or dysregulation of PEF1 and related proteins can lead to various diseases. For instance, PEF1 has been associated with submucosal invasive colon adenocarcinoma . Understanding the structure and function of PEF1 can provide insights into its role in disease mechanisms and potential therapeutic targets.

Recombinant PEF1

Recombinant PEF1 refers to the protein produced through recombinant DNA technology, which involves inserting the PEF1 gene into a suitable expression system to produce the protein in large quantities. This recombinant protein can be used in research to study its structure, function, and interactions with other proteins. It is also valuable in developing assays for drug discovery and understanding disease mechanisms.

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