PECR Human

Peroxisomal Trans-2-enoyl-CoA Reductase Human Recombinant
Cat. No.
BT19843
Source
E.coli.
Synonyms
Peroxisomal trans-2-enoyl-CoA reductase, TERP, HSA250303, SDR29C1, 2,4-dienoyl-CoA reductase-related protein, DCR-RP, pVI-ARL, EC 1.3.1.38, HPDHASE, putative short chain alcohol dehydrogenase, short chain dehydrogenase/reductase family 29C member 1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PECR Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 327 amino acids (1-303) and having a molecular mass of 35.1 kDa.
PECR is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The enzyme PECR is considered to be central to the predicted peroxisomal chain elongation pathway. Its expression is highest in the kidney and liver.
Description
Recombinant human PECR, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 327 amino acids (residues 1-303) and possesses a molecular weight of 35.1 kDa. A 24 amino acid His-tag is fused to the N-terminus of PECR. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The PECR solution (concentration: 1mg/ml) is buffered with 20mM Tris-HCl (pH 8.0) and further contains 0.15M NaCl, 2mM DTT, and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the entire vial can be stored at 4°C. For extended storage, freeze the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
Purity exceeds 95% as assessed by SDS-PAGE analysis.
Synonyms
Peroxisomal trans-2-enoyl-CoA reductase, TERP, HSA250303, SDR29C1, 2,4-dienoyl-CoA reductase-related protein, DCR-RP, pVI-ARL, EC 1.3.1.38, HPDHASE, putative short chain alcohol dehydrogenase, short chain dehydrogenase/reductase family 29C member 1.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMASWAK GRSYLAPGLL QGQVAIVTGG ATGIGKAIVK ELLELGSNVV IASRKLERLK SAADELQANL PPTKQARVIP IQCNIRNEEE VNNLVKSTLD TFGKINFLVN NGGGQFLSPA EHISSKGWHA VLETNLTGTF YMCKAVYSSW MKEHGGSIVN IIVPTKAGFP LAVHSGAARA GVYNLTKSLA LEWACSGIRI NCVAPGVIYS QTAVENYGSW GQSFFEGSFQ KIPAKRIGVP EEVSSVVCFL LSPAASFITG QSVDVDGGRS LYTHSYEVPD HDNWPKGAGD LSVVKKMKET FKEKAKL

Product Science Overview

Gene and Protein Structure

The PECR gene encodes a protein that consists of 303 amino acids and has a molecular mass of approximately 33 kDa . The protein contains a C-terminal type I peroxisomal targeting signal (AKL), which is essential for its localization to the peroxisome . The enzyme is a member of the short-chain dehydrogenase/reductase (SDR) family and is highly conserved across species, sharing 71-75% amino acid identity with its homologs in guinea pig and mouse .

Function and Mechanism

PECR is involved in the chain elongation of fatty acids within the peroxisome . It catalyzes the reduction of trans-2-enoyl-CoAs of varying chain lengths, with maximum activity observed with 10:1 CoA . This reduction step is NADPH-specific, meaning that the enzyme uses NADPH as a cofactor to carry out the reduction reaction . The enzyme does not exhibit 2,4-dienoyl-CoA reductase activity .

Biological Significance

The activity of PECR is essential for the proper functioning of the peroxisomal fatty acid β-oxidation pathway, which is crucial for the metabolism of very-long-chain fatty acids (VLCFAs) . Disruptions in this pathway can lead to the accumulation of VLCFAs, which are associated with various metabolic disorders .

Clinical Relevance

Mutations or dysregulation of the PECR gene have been linked to several diseases, including benign peritoneal mesothelioma and autism spectrum disorder . Understanding the function and regulation of PECR is therefore important for developing therapeutic strategies for these conditions.

Research and Applications

Recombinant human PECR is used in research to study its enzymatic properties and to develop potential therapeutic interventions . The recombinant form of the enzyme is produced by expressing the PECR gene in suitable host cells, followed by purification of the protein . This allows researchers to investigate the enzyme’s structure, function, and role in various metabolic pathways.

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