PDXK Human
Description
Product Specs
MGSSHHHHHH SSGLVPRGSH MGSHMEEECR VLSIQSHVIR GYVGNRAATF PLQVLGFEID AVNSVQFSNH TGYAHWKGQV LNSDELQELY EGLRLNNMNK YDYVLTGYTR DKSFLAMVVD IVQELKQQNP RLVYVCDPVL GDKWDGEGSM YVPEDLLPVY KEKVVPLADI ITPNQFEAELLSGRKIHSQE EALRVMDMLH SMGPDTVVIT SSDLPSPQGS NYLIVLGSQR RRNPAGSVVM ERIRMDIRKV DAVFVGTGDL FAAMLLAWTH KHPNNLKVAC EKTVSTLHHV LQRTIQCAKA QAGEGVRPSP MQLELRMVQS KRDIEDPEIV VQATVL.
Product Science Overview
Pyridoxal kinase (PDXK) is an enzyme that plays a crucial role in the metabolism of vitamin B6. It is responsible for the phosphorylation of vitamin B6 vitamers, including pyridoxal, pyridoxine, and pyridoxamine, converting them into their active form, pyridoxal-5-phosphate (PLP). PLP is an essential cofactor for over 140 different enzymatic reactions in intermediary metabolism. The human recombinant form of pyridoxal kinase is produced through recombinant DNA technology, allowing for its study and application in various research and therapeutic contexts.
The preparation of human recombinant pyridoxal kinase involves several steps:
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cDNA Cloning: The gene encoding human pyridoxal kinase is cloned into an appropriate expression vector. This is typically achieved by isolating the cDNA from human tissues and inserting it into a plasmid vector.
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Expression in Host Cells: The recombinant plasmid is introduced into a suitable host cell, such as Escherichia coli (E. coli) or human embryonic kidney (HEK) cells. These cells are then cultured under conditions that promote the expression of the pyridoxal kinase protein.
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Protein Purification: The expressed pyridoxal kinase is purified from the host cells using various chromatographic techniques. This may include affinity chromatography, ion-exchange chromatography, and size-exclusion chromatography. The purified protein is then analyzed for its activity and purity.
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Characterization: The recombinant pyridoxal kinase is characterized to confirm its identity and functionality. This involves determining its molecular weight, enzymatic activity, and kinetic parameters. Additionally, the protein’s structure may be analyzed using techniques such as X-ray crystallography or nuclear magnetic resonance (NMR) spectroscopy .
Pyridoxal kinase catalyzes the following chemical reaction:
[ \text{ATP} + \text{pyridoxal} \rightleftharpoons \text{ADP} + \text{pyridoxal-5’-phosphate (PLP)} ]
In this reaction, ATP and pyridoxal are the substrates, while ADP and pyridoxal-5’-phosphate are the products. This phosphorylation reaction is essential for the conversion of vitamin B6 to its active form, PLP. PLP serves as a coenzyme in various enzymatic processes, including decarboxylation, deamination, transamination, and racemization .
Pyridoxal kinase is ubiquitously expressed in human tissues, with particularly high expression in the testes. The enzyme functions as a homodimer in vivo and is localized in the cytoplasm. The activity of pyridoxal kinase is crucial for maintaining PLP homeostasis, which is vital for numerous metabolic pathways. Deficiencies in pyridoxal kinase activity can lead to various metabolic disorders, including hereditary motor and sensory neuropathy and ceroid lipofuscinosis .
