PDX-1 antibody

The Pancreas Duodenum Homeobox-1 (PDX-1) protein, also known as insulin promoter factor 1 (IPF1), is a critical transcription factor involved in the regulation of insulin gene expression and pancreatic development. The polyclonal rabbit antibody against PDX-1 is widely used in research to study the protein’s role in various biological processes, including diabetes and pancreatic function.

PDX-1 is a homeodomain-containing transcription factor that plays a pivotal role in the development of the pancreas and the regulation of insulin gene transcription in pancreatic beta-cells . It is expressed in all cells during the early stages of development and is mainly restricted to the pancreas and duodenum in adults . PDX-1 is essential for the proper formation of the pancreas, and its absence can lead to pancreatic agenesis .

PDX-1 has been identified as a novel beta-cell-specific autoantigen for type 1 diabetes (T1D) . Autoantibodies against PDX-1 (PAAs) have been detected in non-obese diabetic (NOD) mice and human T1D patients . These autoantibodies are present before the onset of clinically overt diabetes, suggesting that PDX-1 plays a significant role in the autoimmune response leading to T1D .

The polyclonal rabbit antibody against PDX-1 is generated by immunizing rabbits with a peptide corresponding to a specific region of the PDX-1 protein. This antibody is used in various applications, including Western blotting, immunohistochemistry, and ELISA . It has been validated for reactivity with human, mouse, and rat samples .

• Western Blotting: The PDX-1 antibody is used to detect the presence of PDX-1 protein in cell lysates. It is typically used at a dilution of 1:500-1:1000 .
• Immunohistochemistry: This antibody is used to stain tissue sections to visualize the localization of PDX-1 in different tissues .
• ELISA: The antibody can be used in enzyme-linked immunosorbent assays to quantify the amount of PDX-1 protein in various samples .