PDLIM1 Human

PDZ And LIM Domain 1 Human Recombinant
Cat. No.
BT6004
Source
Escherichia Coli.
Synonyms
PDZ And LIM Domain Protein 1, Carboxyl Terminal LIM Domain Protein 1, Epididymis Secretory Protein Li 112m, C-Terminal LIM Domain Protein 1, LIM Domain Protein CLP-36, hCLIM1, HEL-S-112, CLP-36, Elfin.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PDLIM1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 354 amino acids (1-329) and having a molecular mass of 38.7 kDa. PDLIM1 is fused to a 25 amino acid His-tag at N-terminus.

Product Specs

Introduction
PDLIM1 is a cytoplasmic protein that links to the cytoskeleton and belongs to the enigma protein family. It contains two protein-interacting domains: a PDZ domain at the amino-terminal end and one to three LIM domains at the carboxyl-terminal end. PDLIM1 facilitates the interaction of other LIM-interacting proteins with the cytoskeleton. Pseudogenes associated with PDLIM1 are located on chromosomes 3, 14, and 17.
Description
Recombinant human PDLIM1, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 354 amino acids (residues 1-329). It has a molecular weight of 38.7 kDa. The N-terminus of PDLIM1 is fused to a 25 amino acid His-tag.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The PDLIM1 solution is supplied at a concentration of 1 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 1 mM DTT, 2 mM EDTA, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the product is determined to be greater than 85% by SDS-PAGE analysis.
Synonyms
PDZ And LIM Domain Protein 1, Carboxyl Terminal LIM Domain Protein 1, Epididymis Secretory Protein Li 112m, C-Terminal LIM Domain Protein 1, LIM Domain Protein CLP-36, hCLIM1, HEL-S-112, CLP-36, Elfin.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSEFMTTQQ IDLQGPGPWG FRLVGGKDFE QPLAISRVTP GSKAALANLC IGDVITAIDG ENTSNMTHLE AQNRIKGCTD NLTLTVARSE HKVWSPLVTE EGKRHPYKMN LASEPQEVLH IGSAHNRSAM PFTASPASST TARVITNQYN NPAGLYSSEN ISNFNNALES KTAASGVEAN SRPLDHAQPP SSLVIDKESE VYKMLQEKQE LNEPPKQSTS FLVLQEILES EEKGDPNKPS GFRSVKAPVT KVAASIGNAQ KLPMCDKCGT GIVGVFVKLR DRHRHPECYV CTDCGTNLKQ KGHFFVEDQI YCEKHARERV TPPEGYEVVT VFPK

Product Science Overview

Introduction

PDZ and LIM Domain 1 (PDLIM1) is a protein encoded by the PDLIM1 gene in humans. This protein is part of the PDZ-LIM family, which is known for its role in various cellular processes, including signal transduction, cell differentiation, and cytoskeletal organization .

Structure and Function

PDLIM1 contains two key structural domains:

  1. PDZ Domain: This domain is involved in protein-protein interactions and is crucial for the localization of proteins to specific subcellular compartments.
  2. LIM Domain: This domain is involved in protein-protein interactions and is often found in proteins that play roles in cytoskeletal organization and cellular signaling .

PDLIM1 acts as a scaffolding protein, meaning it helps organize and stabilize complexes of proteins, facilitating efficient signal transduction within the cell. This protein is evolutionarily conserved and found across different species, highlighting its fundamental role in cellular functions .

Role in Disease

PDLIM1 has been implicated in various diseases, particularly in cancer. Dysregulation of PDLIM1 can lead to abnormal cell proliferation, migration, and invasion, contributing to tumor development and progression . Additionally, mutations or alterations in the PDLIM1 gene have been associated with conditions such as supravalvular aortic stenosis and Williams-Beuren syndrome .

Research and Applications

Human recombinant PDLIM1 is used in research to study its function and role in disease. Recombinant proteins are produced through recombinant DNA technology, allowing scientists to investigate the protein in a controlled environment. This research can provide insights into the molecular mechanisms underlying various diseases and potentially lead to the development of targeted therapies .

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