PDIA3 Antibody
Protein Disulfide Isomerase A3, Mouse Anti Human
Cat. No.
BT22136
Source
Synonyms
ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC,
ER60, Protein disulfide-isomerase A3, Disulfide isomerase ER-60, Endoplasmic
reticulum resident protein 60, ER protein 60, 58 kDa microsomal protein,
Endoplasmic reticulum resident protein 57, ER protein 57, 58 kDa
glucose-regulated protein, PDIA3.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
Product Specs
Introduction
PDIA3, also known as ERp57, is an enzyme primarily located in the endoplasmic reticulum (ER). It plays a crucial role in protein folding and quality control within the ER. PDIA3 interacts with chaperones like calreticulin and calnexin to facilitate the proper folding of newly synthesized glycoproteins. Its protein disulfide isomerase activity is essential for forming correct disulfide bonds in these proteins. PDIA3 is involved in various cellular processes, including immune response, glucose metabolism, and response to cellular stress. Its role in the major histocompatibility complex (MHC) class I peptide-loading complex highlights its importance in antigen presentation.
Physical Appearance
Sterile, colorless, and clear solution.
Formulation
The antibody is supplied as a 1 mg/ml solution in a buffer containing PBS at pH 7.4, 10% glycerol, and 0.02% sodium azide.
Storage Procedures
For short-term storage (up to 1 month), the antibody can be stored at 4°C. For long-term storage, it is recommended to store the antibody at -20°C. Avoid repeated freeze-thaw cycles to maintain antibody stability and activity.
Stability / Shelf Life
The antibody is stable for 12 months when stored at -20°C and for 1 month when stored at 4°C.
Applications
This PDIA3 antibody has been validated for use in various applications, including ELISA, Western blot analysis, Flow cytometry, and Immunocytochemistry/Immunofluorescence (ICC/IF). It demonstrates high specificity and reactivity towards the target protein. However, optimal working dilutions should be determined for each application through titration to achieve the best results.
Synonyms
ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC,
ER60, Protein disulfide-isomerase A3, Disulfide isomerase ER-60, Endoplasmic
reticulum resident protein 60, ER protein 60, 58 kDa microsomal protein,
Endoplasmic reticulum resident protein 57, ER protein 57, 58 kDa
glucose-regulated protein, PDIA3.
Purification Method
PDIA3 antibody was purified from mouse ascitic fluids by
protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT9E9AT.
Immunogen
Anti-human PDIA3 mAb, is
derived from hybridization of mouse F0 myeloma cells with spleen cells from
BALB/c mice immunized with a recombinant human PDIA3 protein 25-505 amino
acids purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and k light chain.
Product Science Overview
Mouse Anti-Human Antibodies
Mouse anti-human antibodies are secondary antibodies generated by immunizing mice with human antigens. These antibodies are used in various research and diagnostic applications to detect, sort, or purify human proteins. They are often conjugated with enzymes or fluorophores to facilitate detection in techniques such as ELISA, Western blotting, and immunohistochemistry .
Applications
- Detection: Used to identify human proteins in various assays.
- Sorting: Helps in the separation of human cells or proteins from a mixture.
- Purification: Facilitates the isolation of specific human proteins for further study .
Considerations
When using mouse anti-human antibodies, it is essential to be aware of the potential for the Human Anti-Mouse Antibody (HAMA) response. This response can occur when human patients develop antibodies against the mouse antibodies, leading to allergic reactions ranging from mild rashes to severe complications .
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