PDGFRB Human
Sf9, Insect cells.
Platelet-derived growth factor receptor beta, PDGF-R-beta, PDGFR-beta, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, Platelet-derived growth factor receptor 1, PDGFR-1, CD140b, PDGFRB, Beta Platelet-Derived Growth Factor Receptor, Activated Tyrosine Kinase PDGFRB, CD140b Antigen
NDEL1-PDGFRB, EC 2.7.10, CD140B, IBGC4, JTK12, PENTT, IMF1, KOGS, Platelet Derived Growth Factor Receptor Beta, Platelet-Derived Growth Factor Receptor, Beta Polypeptide, Beta-Type Platelet-Derived Growth Factor Receptor, Platelet-Derived Growth Factor Receptor 1, CD140, Antigen-Like Family Member B, PDGF-R-Beta, EC 2.7.10.1, PDGFR-Beta, PDGFR-1, PDGFR1, PDGFR,
Platelet-Derived Growth Factor Receptor Beta.
Sterile filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
PDGFRB produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 739 amino acids (33-532a.a.) and having a molecular mass of 83.3kDa (Molecular size on SDS-PAGE will appear at approximately 100-150kDa).
PDGFRB is expressed with an 239 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Platelet-derived growth factor receptor beta (PDGFRB) is a member of the class III subfamily of receptor tyrosine kinases (RTKs), which also includes receptors for Flt3-ligand, SCF, and M-CSF. PDGFRB plays a crucial role in blood vessel development by stimulating the growth, movement, and recruitment of pericytes and smooth muscle cells to endothelial cells. Additionally, PDGFRB contributes to the reorganization of the actin cytoskeleton and the formation of membrane ruffles. PDGFRB phosphorylates various proteins, including NCK1, PIK3R1, PTPN11, CBL, SHC1, RASA1/GAP, and PLCG1.
Produced in Sf9 insect cells, PDGFRB is a single, glycosylated polypeptide chain consisting of 739 amino acids (33-532a.a.). It has a molecular mass of 83.3 kDa. However, on SDS-PAGE, its molecular size appears to be between 100-150 kDa. This PDGFRB protein is expressed with a 239 amino acid hIgG-His tag at the C-terminus and is purified using proprietary chromatographic techniques.
The product is a sterile-filtered solution that is colorless.
The PDGFRB protein solution has a concentration of 0.25 mg/ml. It is supplied in a buffer containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For longer storage, it is recommended to freeze the product at -20°C. To further enhance stability during long-term storage, consider adding a carrier protein like HSA or BSA (0.1%). It is important to avoid repeated freezing and thawing of the product.
The purity of PDGFRB is determined by SDS-PAGE analysis and is found to be greater than 90.0%.
Platelet-derived growth factor receptor beta, PDGF-R-beta, PDGFR-beta, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, Platelet-derived growth factor receptor 1, PDGFR-1, CD140b, PDGFRB, Beta Platelet-Derived Growth Factor Receptor, Activated Tyrosine Kinase PDGFRB, CD140b Antigen
NDEL1-PDGFRB, EC 2.7.10, CD140B, IBGC4, JTK12, PENTT, IMF1, KOGS, Platelet Derived Growth Factor Receptor Beta, Platelet-Derived Growth Factor Receptor, Beta Polypeptide, Beta-Type Platelet-Derived Growth Factor Receptor, Platelet-Derived Growth Factor Receptor 1, CD140, Antigen-Like Family Member B, PDGF-R-Beta, EC 2.7.10.1, PDGFR-Beta, PDGFR-1, PDGFR1, PDGFR,
Platelet-Derived Growth Factor Receptor Beta.
Sf9, Insect cells.
LVVTPPGPEL VLNVSSTFVL TCSGSAPVVW ERMSQEPPQE MAKAQDGTFS SVLTLTNLTG LDTGEYFCTH NDSRGLETDE RKRLYIFVPD PTVGFLPNDA EELFIFLTEI TEITIPCRVT DPQLVVTLHE KKGDVALPVP YDHQRGFSGI FEDRSYICKT TIGDREVDSD AYYVYRLQVS SINVSVNAVQ TVVRQGENIT LMCIVIGNEV VNFEWTYPRK ESGRLVEPVT DFLLDMPYHI RSILHIPSAE LEDSGTYTCN VTESVNDHQD EKAINITVVE SGYVRLLGEV GTLQFAELHR SRTLQVVFEA YPPPTVLWFK DNRTLGDSSA GEIALSTRNV SETRYVSELT LVRVKVAEAG HYTMRAFHED AEVQLSFQLQ INVPVRVLEL SESHPDSGEQ TVRCRGRGMP QPNIIWSACR DLKRCPRELP PTLLGNSSEE ESQLETNVTY WEEEQEFEVV STLRLQHVDR PLSVRCTLRN AVGQDTQEVI VVPHSLPFKV LEPKSCDKTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS RDELTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGKHHHHHH.
Product Science Overview
Platelet-Derived Growth Factor Receptor, Beta (PDGFR-β) is a transmembrane glycoprotein that belongs to the receptor tyrosine kinase family. It plays a crucial role in various cellular processes, including cell proliferation, differentiation, and growth. The receptor is activated by binding to its ligand, Platelet-Derived Growth Factor (PDGF), leading to receptor dimerization and subsequent phosphorylation of tyrosine residues .
PDGFR-β is encoded by the PDGFRB gene, which is located on chromosome 5. The receptor consists of an extracellular ligand-binding domain, a single transmembrane helix, and an intracellular tyrosine kinase domain. Upon ligand binding, PDGFR-β undergoes dimerization, which can occur either as a homodimer (PDGFB or PDGFD) or a heterodimer (PDGFA and PDGFB). This dimerization is essential for the activation of the receptor’s kinase activity .
The primary function of PDGFR-β is to regulate cell proliferation and differentiation, particularly in cells of mesenchymal origin. It is also involved in the rearrangement of the actin cytoskeleton, which is crucial for cell migration and shape changes .
PDGFR-β is essential for the normal development of the cardiovascular system. It aids in the formation of blood vessels and the development of smooth muscle cells. Abnormalities in PDGFR-β signaling can lead to various developmental disorders and diseases .
One of the significant implications of PDGFR-β dysregulation is its role in cancer. Overexpression or mutations in PDGFR-β have been associated with several types of cancers, including gliomas and sarcomas. In pediatric high-grade gliomas, PDGFR-β acts as a potent inflammatory driver, contributing to the tumor’s malignancy .
