PDGFD Human
Platelet Derived Growth Factor-D Human Recombinant
Cat. No.
BT27551
Source
E.coli.
Synonyms
Platelet Derived Growth Factor D, Spinal Cord-Derived Growth Factor B, Iris-Expressed Growth Factor, SCDGF-B, IEGF, PDGF-D, MSTP036.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
PDGFD Human Recombinant produced in E. coli is a single polypeptide chain containing 146 amino acids (250-370) and having a molecular mass of 16.6 kDa.
PDGFD is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
PDGFD is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Platelet-derived growth factor D (PDGFD) is a member of the platelet-derived growth factor family. Unlike other family members, PDGFD only forms homodimers. It plays a crucial role in wound healing by promoting macrophage recruitment, increasing interstitial pressure, and supporting blood vessel maturation during angiogenesis. PDGFD can also trigger events leading to mesangial proliferative glomerulonephritis, including the influx of monocytes and macrophages and the production of extracellular matrix. The PDGF family consists of four members, all of which are mitogenic factors for cells of mesenchymal origin and share a core motif of eight cysteines. PDGFD distinguishes itself from the alpha and beta members by possessing a unique N-terminal CUB domain.
Description
Recombinant human PDGFD, produced in E. coli, is a single polypeptide chain with a molecular weight of 16.6 kDa. It encompasses amino acids 250-370 and includes a 25 amino acid His-tag at the N-terminus. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The PDGFD solution is provided at a concentration of 1mg/ml and contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea, and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), keep at 4°C. For long-term storage, freeze at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for extended storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of PDGFD is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
Platelet Derived Growth Factor D, Spinal Cord-Derived Growth Factor B, Iris-Expressed Growth Factor, SCDGF-B, IEGF, PDGF-D, MSTP036.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMSYHDR KSKVDLDRLN DDAKRYSCTP RNYSVNIREE LKLANVVFFP RCLLVQRCGG NCGCGTVNWR SCTCNSGKTV KKYHEVLQFE PGHIKRRGRA KTMALVDIQL DHHERCDCIC SSRPPR.
Product Science Overview
Introduction
Platelet-Derived Growth Factor-D (PDGF-D) is a member of the PDGF family, which plays a crucial role in regulating cell growth, proliferation, and angiogenesis. PDGF-D, like other PDGFs, is a potent mitogen for cells of mesenchymal origin, including fibroblasts, smooth muscle cells, and glial cells .
Structure and Function
PDGF-D is a dimeric glycoprotein composed of two disulfide-linked subunits. It is one of the five known isoforms of PDGF, which include PDGF-AA, PDGF-BB, PDGF-CC, PDGF-DD, and the heterodimer PDGF-AB . PDGF-D specifically binds to the PDGFRβ receptor, which is a receptor tyrosine kinase (RTK). Upon binding, the receptor dimerizes and undergoes autophosphorylation, activating various downstream signaling pathways such as the PI3K pathway and the STAT3 pathway .
Biological Role
PDGF-D is involved in several critical biological processes:
- Angiogenesis: It promotes the formation of new blood vessels from pre-existing ones, which is essential for tissue growth and repair .
- Cell Proliferation: PDGF-D stimulates the proliferation of mesenchymal cells, contributing to tissue regeneration and repair .
- Chemotaxis: It directs the migration of mesenchymal cells to sites of injury or inflammation .
Recombinant PDGF-D
Applications
Recombinant PDGF-D has several applications in medicine and research:
- Wound Healing: It is used to promote the healing of chronic ulcers and other wounds by stimulating cell proliferation and angiogenesis .
- Orthopedic Surgery: PDGF-D is used to stimulate bone regeneration and repair, serving as an alternative to bone autografts .
- Periodontics: It aids in the regeneration of periodontal tissues, improving outcomes in dental surgeries .
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