PDCL3 Antibody

Phosducin-like 3 (PDCL3) is a member of the phosducin-like protein family, which is known for its role in binding to the beta-gamma subunits of G proteins. PDCL3 shares a significant amino acid sequence homology with phosducin and is considered a potential regulator of heterotrimeric G proteins . This protein is characterized by a thioredoxin-like structural domain and evolutionary conservation .

The mouse anti-human PDCL3 monoclonal antibody is derived from the hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PDCL3 protein. The protein used for immunization is typically purified from E. coli and spans 1-239 amino acids . The antibody is purified from mouse ascitic fluids using protein-A affinity chromatography .

PDCL3 plays a crucial role in various biological processes, including angiogenesis and apoptosis . It has been identified as a prognostic biomarker associated with immune infiltration in hepatocellular carcinoma (LIHC). High PDCL3 expression is linked to poorer clinical staging and prognosis in LIHC . Additionally, PDCL3 is involved in modulating immune responses and has positive correlations with multiple immune checkpoint genes .

PDCL3 regulates the biological functions of LIHC by modulating immune infiltration. High PDCL3 expression in LIHC decreases tumor immune activity by reducing macrophage infiltration . This protein also promotes LIHC cell proliferation, migration, invasion, and colony-forming ability .

The precise regulatory mechanisms of PDCL3 in cancer are still under investigation. However, enrichment analysis of PDCL3-associated genes has revealed its involvement in various immune responses . Further research is needed to fully understand the regulatory pathways and mechanisms through which PDCL3 exerts its effects in different cancer types.

The PDCL3 antibody has been tested by ELISA and Western blot analysis to ensure specificity and reactivity . It is recommended for laboratory research use only and should not be used as drugs, agricultural or pesticidal products, food additives, or household chemicals .

For optimal storage, the PDCL3 antibody should be kept at 4°C for up to one month or at -20°C for longer periods. It is important to prevent freeze-thaw cycles to maintain the antibody’s stability and shelf life .