PCYT2 Human

Phosphate Cytidylyltransferase 2 Human Recombinant
Cat. No.
BT11941
Source
Escherichia Coli.
Synonyms

MEA-phosphate cytidylyltransferase, CTP:phospho MEA cytidylyltransferase, Phosphoryl MEA transferase, PCYT2, ET.

Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PCYT2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 409 amino acids (1-389) and having a molecular mass of 45.9kDa.
PCYT2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
PCYT2, a member of the cytidylyltransferase family, is an enzyme that plays a crucial role in phospholipid synthesis. Specifically, it catalyzes the formation of CDP-MEA from CTP and phospho MEA within the Kennedy pathway. PCYT2 exhibits its highest expression levels in the liver, heart, and skeletal muscle.
Description
Recombinant PCYT2, derived from humans and produced in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 409 amino acids (specifically, amino acids 1 to 389) and exhibits a molecular weight of 45.9 kDa. The protein includes a 20-amino acid His-tag fused to its N-terminus. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
The product appears as a sterile, colorless solution after filtration.
Formulation
The PCYT2 solution has a concentration of 0.25 mg/ml and contains the following components: 20 mM Tris-HCl buffer at a pH of 8.0, 20% glycerol, 0.1 M NaCl, 1 mM DTT, and 0.1 mM PMSF.
Stability
For optimal storage, the product should be kept at 4°C if it will be used within 2-4 weeks. For extended storage, it is recommended to store the product frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. To maintain product integrity, avoid repeated cycles of freezing and thawing.
Purity
The purity of the product exceeds 90.0%, as determined by SDS-PAGE analysis.
Synonyms

MEA-phosphate cytidylyltransferase, CTP:phospho MEA cytidylyltransferase, Phosphoryl MEA transferase, PCYT2, ET.

Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MIRNGRGAAG GAEQPGPGGR RAVRVWCDGC YDMVHYGHSN QLRQARAMGD YLIVGVHTDE EIAKHKGPPV FTQEERYKMV QAIKWVDEVV PAAPYVTTLE TLDKYNCDFC VHGNDITLTV DGRDTYEEVK QAGRYRECKR TQGVSTTDLV GRMLLVTKAH HSSQEMSSEY REYADSFGKC PGGRNPWTGV SQFLQTSQKI IQFASGKEPQ PGETVIYVAG AFDLFHIGHV DFLEKVHRLA ERPYIIAGLH FDQEVNHYKG KNYPIMNLHE RTLSVLACRY VSEVVIGAPY AVTAELLSHF KVDLVCHGKT EIIPDRDGSD PYQEPKRRGI FRQIDSGSNL TTDLIVQRII TNRLEYEARN QKKEAKELAF LEAARQQAAQ PLGERDGDF.

Product Science Overview

Structure and Expression

The recombinant human PCYT2 protein is typically expressed in Escherichia coli and is purified to a high degree of purity, often exceeding 90% . The protein consists of 389 amino acids and is often tagged with a His-tag at the N-terminus to facilitate purification . The molecular weight of the recombinant protein is approximately 45.9 kDa .

Function and Biological Significance

PCYT2 is essential for the synthesis of phosphatidylethanolamine, which is a dominant inner-leaflet phospholipid in cell membranes . Phosphatidylethanolamine plays a critical role in various cellular processes, including:

  • Membrane Function: It structurally stabilizes membrane-anchored proteins.
  • Cell Division and Fusion: It is involved in the processes of cell division and cell fusion.
  • Blood Coagulation and Apoptosis: It participates in blood coagulation and programmed cell death (apoptosis) .
Clinical Relevance

Mutations or dysregulation of the PCYT2 gene have been associated with certain diseases, including Spastic Paraplegia 82, Autosomal Recessive, and Hereditary Spastic Paraplegia . These conditions highlight the importance of PCYT2 in maintaining normal cellular functions and its potential as a therapeutic target.

Applications

Recombinant human PCYT2 protein is widely used in research to study its function and role in phospholipid biosynthesis. It is also utilized in various biochemical assays, including SDS-PAGE and mass spectrometry (MS), to investigate its properties and interactions .

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