PCNA Human, Sf9

The PCNA gene is located on human chromosome 20p12.3 and encodes a protein with a molecular weight of approximately 29.8 kDa . The protein is expressed in the nucleus and is involved in various cellular processes, including DNA synthesis, repair, and cell cycle regulation .

PCNA acts as a sliding clamp, providing a stable platform for DNA polymerases and other proteins involved in DNA replication and repair . It binds to a variety of nuclear proteins, organizing biochemical processes at the DNA replication fork . During the cell cycle, PCNA expression peaks during the S-phase, when DNA synthesis occurs, and declines during the G2 and M phases .

Recombinant PCNA is often produced using the baculovirus expression system in Sf9 insect cells. This system allows for high-level expression of the protein with proper folding and post-translational modifications . The recombinant PCNA is typically tagged with a 6x His tag to facilitate purification using chromatographic techniques .

Recombinant PCNA is widely used in research to study DNA replication and repair mechanisms. It is also used in assays to identify potential inhibitors of PCNA, which could be valuable in cancer therapy . The protein’s role in maintaining genome stability makes it a critical target for understanding and potentially treating various diseases, including cancer .

Recombinant PCNA is usually supplied in a liquid form and should be stored at -70°C to maintain its stability . It is important to avoid freeze-thaw cycles to prevent protein degradation .