PCMTD1 Human
Protein-L-Isoaspartate O-Methyltransferase 1 Human Recombinant
Cat. No.
BT5192
Source
Escherichia Coli.
Synonyms
Protein-L-isoaspartate O-methyltransferase domain-containing protein 1, PCMTD1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
PCMTD1 Human Recombinant produced in E. coli is. a single polypeptide chain containing 243 amino acids (1-220) and having a molecular mass of 27kDa. PCMTD1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Protein-L-Isoaspartate O-Methyltransferase 1, also called PCMTD1, belongs to the methyltransferase superfamily. It is found in the cytoplasm.
Description
Recombinant human PCMTD1, expressed in E. coli, is a single polypeptide chain with a molecular weight of 27kDa. It consists of 243 amino acids, with residues 1-220 corresponding to PCMTD1 and a 23 amino acid His-tag fused at the N-terminus. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
The PCMTD1 solution is provided at a concentration of 0.25mg/ml in a buffer containing 20mM Tris-HCl (pH 8.0), 20% glycerol, 0.15M NaCl, and 1mM DTT.
Stability
For short-term storage (up to 4 weeks), store the entire vial at 4°C. For long-term storage, freeze the product at -20°C. Adding a carrier protein such as 0.1% HSA or BSA is recommended for extended storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is greater than 95% as assessed by SDS-PAGE.
Synonyms
Protein-L-isoaspartate O-methyltransferase domain-containing protein 1, PCMTD1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMGGAVSA GEDNDDLIDN LKEAQYIRTE RVEQAFRAID RGDYYLEGYR DNAYKDLAWK HGNIHLSAPC IYSEVMEALK LQPGLSFLNL GSGTGYLSTM VGLILGPFGI NHGIELHSDV VEYAKEKLES FIKNSDSFDK FEFCEPAFVV GNCLQIASDS HQYDRIYCGA GVQKDHENYM KILLKVGGIL VMPIEDQLTQ IMRTGQNTWE SKNILAVSFA PLV.
Product Science Overview
Function and Mechanism
PCMT1 plays a vital role in protein repair by recognizing and converting abnormal D-aspartyl and L-isoaspartyl residues back to their normal L-aspartyl form . These abnormal residues are typically formed due to spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues in aging peptides and proteins . The enzyme catalyzes the methyl esterification of these residues, initiating their repair .
The reaction catalyzed by PCMT1 can be summarized as follows:
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