PCK1 Human
Description
PCK1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Phosphoenolpyruvate carboxykinase 1 (PCK1) is a key regulator of gluconeogenesis, the process of glucose production. It plays a crucial role in stimulating hepatic glucose production. PCK1 expression is influenced by factors such as cAMP, glucocorticoids, and diet. Modulating the signals that control PCK1 levels represents a potential therapeutic strategy for managing diabetes and obesity.
Repeated freezing and thawing should be avoided.
Product Science Overview
Phosphoenolpyruvate Carboxykinase 1 (PCK1), also known as PEPCK-C, is a crucial enzyme in the metabolic pathway of gluconeogenesis. This pathway is responsible for the synthesis of glucose from non-carbohydrate precursors, which is essential for maintaining blood glucose levels, especially during fasting or intense exercise .
In humans, PCK1 exists in two isoforms: a cytosolic form (PCK1) and a mitochondrial form (PCK2). The cytosolic form is primarily involved in gluconeogenesis, while the mitochondrial form plays a role in the transport of phosphoenolpyruvate (PEP) across the mitochondrial membrane . The cytosolic form of PCK1 is encoded by the PCK1 gene located on chromosome 20q13.31 .
PCK1 catalyzes the conversion of oxaloacetate to phosphoenolpyruvate and carbon dioxide. This reaction is a key step in gluconeogenesis and is tightly regulated to ensure proper glucose homeostasis . The enzyme’s activity is influenced by various factors, including hormonal control, which adjusts the transcription of the PCK1 gene in response to metabolic needs .
Recombinant PCK1 is produced using recombinant DNA technology, which involves inserting the PCK1 gene into a suitable expression system, such as bacteria or yeast. This allows for the large-scale production of the enzyme for research and therapeutic purposes. Recombinant PCK1 is used in various studies to understand its role in metabolism and its potential implications in diseases such as diabetes mellitus and cardiovascular diseases .
PCK1 plays a significant role in regulating blood glucose levels. Dysregulation of PCK1 activity can lead to metabolic disorders such as diabetes mellitus. Elevated levels of serum anti-PCK1 antibodies have been associated with diabetes and cardiovascular diseases, making it a potential biomarker for these conditions . Understanding the function and regulation of PCK1 is crucial for developing therapeutic strategies for metabolic diseases.
