PCID2 Human

PCI Domain Containing 2 Human Recombinant
Cat. No.
BT5144
Source
E.coli.
Synonyms
PCI Domain Containing 2, CSN12-Like Protein, PCI Domain-Containing Protein 2, F10, PCI domain-containing protein 2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PCID2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 341 amino acids (80-399 a.a) and having a molecular mass of 39.3kDa.
PCID2 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
PCID2 (PCI Domain Containing 2) is a protein involved in B cell development and cell cycle regulation. It plays a crucial role in early-stage B lymphocytes by regulating the expression of MAD2, a key mitotic checkpoint protein. PCID2 is also a component of the TREX-2 complex, which is essential for mRNA export from the nucleus. TREX-2 facilitates the docking of export-ready messenger ribonucleoprotein particles (mRNPs) to the nuclear pore complex's nuclear basket.
Description
This product consists of a recombinant human PCID2 protein produced in E. coli. It is a single, non-glycosylated polypeptide chain comprising 341 amino acids (specifically, amino acids 80 to 399). The protein has a molecular weight of 39.3 kDa. For purification and detection purposes, a 21 amino acid His-tag is fused to the N-terminus of the protein. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The PCID2 protein is supplied in a solution with a concentration of 1.0 mg/ml. The solution is buffered with 20mM Tris-HCl at a pH of 8.0 and contains 10% glycerol and 0.4M Urea.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. Adding a carrier protein like HSA or BSA (0.1%) can be beneficial for long-term storage. Avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of the PCID2 protein is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms
PCI Domain Containing 2, CSN12-Like Protein, PCI Domain-Containing Protein 2, F10, PCI domain-containing protein 2.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAYKCQTVIV QSFLRAFQAH KEENWALPVM YAVALDLRVF ANNADQQLVK KGKSKVGDML EKAAELLMSC FRVCASDTRA GIEDSKKWGM LFLVNQLFKI YFKINKLHLC KPLIRAIDSS NLKDDYSTAQ RVTYKYYVGR KAMFDSDFKQ AEEYLSFAFE HCHRSSQKNK RMILIYLLPV KMLLGHMPTV ELLKKYHLMQ FAEVTRAVSE GNLLLLHEAL AKHEAFFIRC GIFLILEKLK IITYRNLFKK VYLLLKTHQL SLDAFLVALK FMQVEDVDID EVQCILANLI YMGHVKGYIS HQHQKLVVSK QNPFPPLSTV C.

Product Science Overview

Introduction

PCI Domain Containing 2 (PCID2) is a protein encoded by the PCID2 gene in humans. This protein is a part of the TREX-2 complex, which plays a crucial role in the export of mRNA from the nucleus to the cytoplasm . The recombinant form of this protein is often used in research to study its functions and interactions.

Structure and Function

PCID2 belongs to the CSN12 family and contains a PCI (Proteasome, COP9 signalosome, and eIF3) domain . This domain is essential for the protein’s role in various cellular processes, including mRNA export and cell cycle regulation. The protein has multiple isoforms, ranging from 376 to 453 amino acids in length .

One of the key functions of PCID2 is to regulate the expression of the MAD2L1 protein, which is crucial for the cell cycle checkpoint during B cell differentiation . This regulation is vital for B-cell survival. Additionally, PCID2 binds and stabilizes the BRCA2 protein, which is involved in DNA repair and maintaining genomic stability .

Role in Disease

PCID2 has been linked to several diseases, including breast cancer and malignant acrospiroma . Its interaction with BRCA2 suggests a role in the control of DNA damage and transcription-associated genomic instability, which are critical factors in cancer development .

Recombinant PCID2

Recombinant PCID2 is typically produced in E. coli and is available with various tags, such as a His-tag, to facilitate purification and detection . The recombinant protein is used in various assays to study its function, interactions, and role in different cellular processes.

Research Applications

Research on PCID2 has shown its involvement in several pathways, including the negative regulation of apoptosis, regulation of mRNA stability, and positive regulation of B cell differentiation . It also interacts with several other proteins, such as KPNB1, SMAD2, BRF2, NEK6, and SHFM1, indicating its broad role in cellular functions .

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