PCBP1 Human
Poly (RC) Binding Protein 1 Human Recombinant
Cat. No.
BT5000
Source
Escherichia Coli.
Synonyms
Poly(rC) binding protein 1, hnRNP-E1, hnRNP-X, HNRPE1, HNRPX, Alpha-CP1, Heterogeneous nuclear ribonucleoprotein E1, Nucleic acid-binding protein SUB2.3, PCBP1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
PCBP1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 186 amino acids (1-163) and having a molecular mass of 19.6 kDa.
PCBP1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
PCBP1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
PCBP1, also known as poly(rC)-binding protein 1, is a protein that plays a crucial role in binding single-stranded nucleic acids, particularly oligo dC sequences. It is involved in the formation of a specific complex with alpha-globin mRNA, contributing to its stability. PCBP1, along with PCBP-2 and hnRNPK, constitutes the primary cellular poly(rC)-binding protein. Both PCBP1 and PCBP-2 act as translational coactivators of poliovirus RNA by interacting with stem-loop IV of the IRES in a sequence-specific manner. Moreover, PCBP1 facilitates poliovirus RNA replication through binding to its 5'-terminal cloverleaf structure.
Description
Recombinant PCBP1 from humans has been expressed in E.coli. This single, non-glycosylated polypeptide chain comprises 186 amino acids, spanning residues 1 to 163, and exhibits a molecular weight of 19.6 kDa. The N-terminus of PCBP1 is fused to a 23 amino acid His-tag. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The PCBP1 solution is supplied at a concentration of 1mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 0.15M NaCl, 20% glycerol, and 1mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the PCBP1 vial can be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. To maintain protein integrity, it is crucial to avoid repeated cycles of freezing and thawing.
Purity
The purity of PCBP1 is determined to be greater than 85.0% based on SDS-PAGE analysis.
Synonyms
Poly(rC) binding protein 1, hnRNP-E1, hnRNP-X, HNRPE1, HNRPX, Alpha-CP1, Heterogeneous nuclear ribonucleoprotein E1, Nucleic acid-binding protein SUB2.3, PCBP1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMDAGVTE SGLNVTLTIR LLMHGKEVGS IIGKKGESVK RIREESGARI NISEGNCPER IITLTGPTNA IFKAFAMIID KLEEDINSSM TNSTAASRPP VTLRLVVPAT QCGSLIGKGG CKIKEIREST GAQVQVAGDM LPNSTERAIT IAGVPQSVTE CVKQIC.
Product Science Overview
Structure and Domains
PCBP1 contains three K-homologous (KH) domains, which are crucial for its RNA-binding capabilities . These domains enable PCBP1 to bind single-stranded nucleic acids, particularly oligo dC sequences . The protein is part of a subfamily of heterogeneous nuclear ribonucleoproteins (hnRNPs) characterized by their high affinity for polycytosine (poly ©) sequences .
Functions
PCBP1 is involved in various cellular processes, including:
- RNA Metabolism: It plays a role in mRNA splicing, stability, and translation .
- Translational Control: PCBP1, along with PCBP2, acts as a translational coactivator for poliovirus RNA by interacting with specific RNA structures . It also regulates the translation of other viral RNAs, such as human papillomavirus type 16 L2 mRNA and hepatitis A virus RNA .
- mRNA Stability: PCBP1 is implicated in the formation of a sequence-specific alpha-globin mRNP complex, which is associated with the stability of alpha-globin mRNA .
Clinical Significance
Recombinant PCBP1
Recombinant PCBP1 is produced using recombinant DNA technology, which involves inserting the PCBP1 gene into an expression system, such as bacteria or yeast, to produce the protein in large quantities. This recombinant protein is used in research to study its functions and interactions, as well as in the development of therapeutic applications.
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