PBLD Human

Phenazine Biosynthesis-Like Protein Domain Containing Human Recombinant
Cat. No.
BT4919
Source
Escherichia Coli.
Synonyms
Phenazine biosynthesis-like domain-containing protein, MAWD-binding protein, Unknown protein 32 from 2D-page of liver tissue, PBLD, MAWBP, MAWDBP, FLJ14767, FLJ35507.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PBLD Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 308 amino acids (1-288 a.a.) and having a molecular mass of 33.9kDa. The PBLD is purified by proprietary chromatographic techniques.

Product Specs

Introduction
PBLD, a member of the phenazine biosynthesis-like protein (PhzF) family, is the sole representative of this family found in the human genome. Expressed in a wide range of tissues, PBLD plays a role in the MAPK signaling pathway and is involved in numerous fundamental cellular processes. Notably, its expression is elevated in various disease states, including folate deficiency and hypotension.
Description
Recombinant human PBLD, fused with a 20 amino acid His tag at its N-terminus, is produced in E. coli. This yields a single, non-glycosylated polypeptide chain comprising 308 amino acids (residues 1-288) with a molecular weight of 33.9kDa. Purification of PBLD is achieved through proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The PBLD solution is provided at a concentration of 1 mg/ml and is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 10% glycerol, 2mM DTT, and 0.1M NaCl.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To further enhance long-term stability, the addition of a carrier protein such as HSA or BSA (0.1%) is advised. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 95.0% as determined by SDS-PAGE analysis.
Synonyms
Phenazine biosynthesis-like domain-containing protein, MAWD-binding protein, Unknown protein 32 from 2D-page of liver tissue, PBLD, MAWBP, MAWDBP, FLJ14767, FLJ35507.
Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MKLPIFIADA FTARAFRGNP AAVCLLENEL DEDMHQKIAR EMNLSETAFI RKLHPTDNFA QSSCFGLRWF TPASEVPLCG HATLASAAVL FHKIKNMNST LTFVTLSGEL RARRAEDGIV LDLPLYPAHP QDFHEVEDLI KTAIGNTLVQ DICYSPDTQK LLVRLSDVYN RSFLENLKVN TENLLQVENT GKVKGLILTL KGEPGGQTQA FDFYSRYFAP WVGVAEDPVT GSAHAVLSSY WSQHLGKKEM HAFQCSHRGG ELGISLRPDG RVDIRGGAAV VLEGTLTA.

Product Science Overview

Introduction

Phenazine Biosynthesis-Like Protein Domain Containing (PBLD) is a protein encoded by the PBLD gene in humans. This protein is involved in various biological processes and has been studied for its potential roles in disease mechanisms and therapeutic applications.

Gene and Protein Structure

The PBLD gene is located on chromosome 10 and encodes a protein that consists of 255 amino acids . The protein is highly conserved across different species, indicating its essential biological functions . It shares significant sequence similarity with the Arabidopsis enzyme Phzf, which is involved in phenazine biosynthesis .

Biological Functions

PBLD is known to play a role in several cellular processes, including:

  • Maintenance of Gastrointestinal Epithelium: PBLD is involved in maintaining the integrity of the gastrointestinal lining .
  • Negative Regulation of SMAD Protein Signal Transduction: It negatively regulates the SMAD protein signaling pathway, which is crucial for various cellular processes .
  • Negative Regulation of Transforming Growth Factor Beta (TGF-β) Receptor Signaling Pathway: PBLD also negatively regulates the TGF-β receptor signaling pathway, which is important for cell growth and differentiation .
Disease Associations

Mutations or dysregulation of the PBLD gene have been associated with certain diseases, including Osebold-Remondini Syndrome . This highlights the importance of PBLD in maintaining normal cellular functions and its potential role in disease mechanisms.

Research and Therapeutic Potential

Recent studies have explored the therapeutic potential of targeting PBLD in various diseases. For instance, research has shown that vitexin, a novel VDR agonist, can regulate macrophage polarization through the VDR/PBLD pathway, thereby mitigating the progression from chronic colitis to colorectal cancer . This indicates that PBLD could be a potential target for therapeutic interventions in inflammatory and cancer-related conditions.

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