PAX8 Human

Paired Box 8 Human Recombinant
Cat. No.
BT2562
Source
Escherichia Coli.
Synonyms
Paired Box 8, Paired Box Gene 8, PAX8.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PAX8 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 310 amino acids (1-287 a.a) and having a molecular mass of 33.4kDa.
PAX8 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
PAX8, also referred to as paired box gene 8, is crucial for thyroid follicular cell development and the expression of thyroid-specific genes. This protein forms complexes with transcription factors TTF-1 and TTF-2 to activate thyroid follicular cell differentiation and thyroid hormone production. This regulation is achieved by controlling the expression of genes such as NIS (sodium iodide symporter), TPO (thyroid peroxidase), TG (thyroglobulin), and TSHR (thyrotropin receptor). Mutations in the PAX8 gene are linked to CHNG2, a disorder characterized by congenital hypothyroidism without goiter.
Description
Recombinant Human PAX8 protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 310 amino acids, with a sequence spanning from amino acid 1 to 287, and has a molecular weight of 33.4 kDa. The protein is engineered with a 23 amino acid His-tag fused to its N-terminus. Purification is carried out using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The PAX8 protein solution is provided at a concentration of 0.25 mg/ml and is formulated in a buffer consisting of Phosphate buffered saline (pH 7.4), 20% glycerol, and 1 mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended periods, storage at -20°C is recommended. To ensure long-term stability, adding a carrier protein like HSA or BSA (0.1%) is advisable. Repeated freezing and thawing should be avoided.
Purity
The purity of the protein is determined to be greater than 90% using SDS-PAGE analysis.
Synonyms
Paired Box 8, Paired Box Gene 8, PAX8.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMPHNSIR SGHGGLNQLG GAFVNGRPLP EVVRQRIVDL AHQGVRPCDI SRQLRVSHGC VSKILGRYYE TGSIRPGVIG GSKPKVATPK VVEKIGDYKR QNPTMFAWEI RDRLLAEGVC DNDTVPSVSS INRIIRTKVQ QPFNLPMDSC VATKSLSPGH TLIPSSAVTP PESPQSDSLG STYSINGLLG IAQPGSDKRK MDDSDQDSCR LSIDSQSSSS GPRKHLRTDA FSQHHLEPLE CPFERQHYPE AYASPSHTKG EQEVNTLAMP MATPPTPPTA RPGASPTPAC

Product Science Overview

Structure and Function

PAX8 typically contains:

  • Paired Box Domain: This domain is essential for DNA-binding activity. It consists of 128 amino acids and is folded into two β-sheets and six α-helices divided into two subdomains, PAI and RED. Mutations in these subdomains can disrupt DNA-binding activity and lead to congenital abnormalities .
  • Octapeptide: This is a conserved motif found in PAX proteins.
  • Paired-Type Homeodomain: This domain can serve as a second independent DNA-binding region .
Role in Development and Disease

PAX8 is crucial for the development of several organs, including the thyroid gland, kidneys, and reproductive system. It is involved in:

  • Thyroid Development: PAX8 is essential for the differentiation and function of thyroid follicular cells.
  • Kidney Cell Differentiation: It plays a role in the development of the kidneys.
  • Reproductive System Development: PAX8 is important for the development of the reproductive system, particularly the Müllerian ducts .
PAX8 in Cancer

PAX8 expression is deregulated in several types of cancer, including those arising from the reproductive tract. In cancer, PAX8 can:

  • Stimulate Proliferation: It promotes the growth of cancer cells.
  • Inhibit Apoptosis: PAX8 helps cancer cells evade programmed cell death.
  • Regulate Angiogenesis: It is involved in the formation of new blood vessels to supply the tumor .

PAX8 can interact with different protein partners during cancer progression and may exhibit significant function-altering alternative splicing. Moreover, the expression of PAX8 in cancer can serve as a biomarker for diagnostic and prognostic purposes .

Recombinant PAX8

Recombinant PAX8 is a form of the protein that is produced using recombinant DNA technology. It is typically expressed in Escherichia coli and purified for use in research. The recombinant protein often includes a His-tag for purification purposes and corresponds to specific amino acids of the human PAX8 protein .

Recombinant PAX8 is used in various research applications, including studies on gene regulation, protein-protein interactions, and the role of PAX8 in development and disease. It is also used to investigate the mechanisms of PAX8 function and its potential as a therapeutic target in cancer .

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