PARD6B Human
Par-6 Partitioning Defective 6 Homolog Beta Human Recombinant
Cat. No.
BT4740
Source
Escherichia Coli.
Synonyms
Partitioning defective 6 homolog beta, PAR-6 beta, PAR-6B, PARD6B, PAR6B.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
PARD6B Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 395 amino acids (1-372) and having a molecular mass of 43.6kDa.
PARD6B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
PARD6B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Partitioning defective 6 homolog beta (PARD6B), a member of the PAR6 family, is a protein encoded by the PARD6B gene. This protein plays a critical role in establishing cellular asymmetry, a fundamental process in the development of multicellular organisms. PARD6B contains three key domains: a PSD95/Discs-large/ZO1 (PDZ) domain, an OPR domain, and a semi-Cdc42/Rac interactive binding (CRIB) domain. It interacts with proteins like Cdc42 and Rac, which are involved in cell growth, polarity, and potentially, oncogenic transformation by Ras. PARD6B exhibits expression in various tissues, including the pancreas, adult and fetal kidneys, with weaker expression in the placenta and lungs.
Description
Recombinant human PARD6B, expressed in E. coli, is a single, non-glycosylated polypeptide chain. This protein consists of 395 amino acids, including a 23 amino acid His-tag at the N-terminus, and has a molecular weight of 43.6 kDa. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
A sterile, colorless solution.
Formulation
The PARD6B solution is supplied at a concentration of 0.5 mg/ml and contains 20mM Tris-HCl buffer (pH 8.0), 2M Urea, 20% glycerol, and 0.2M NaCl.
Stability
For short-term storage (up to 2-4 weeks), the solution can be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of the PARD6B protein is determined to be greater than 85.0% using SDS-PAGE analysis.
Synonyms
Partitioning defective 6 homolog beta, PAR-6 beta, PAR-6B, PARD6B, PAR6B.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMNRSHRH GAGSGCLGTM EVKSKFGAEF RRFSLERSKP GKFEEFYGLL QHVHKIPNVD VLVGYADIHG DLLPINNDDN YHKAVSTANP LLRIFIQKKE EADYSAFGTD TLIKKKNVLT NVLRPDNHRK KPHIVISMPQ DFRPVSSIID VDILPETHRR VRLYKYGTEK PLGFYIRDGS SVRVTPHGLE KVPGIFISRL VPGGLAQSTG LLAVNDEVLE VNGIEVSGKS LDQVTDMMIA NSRNLIITVR PANQRNNVVR NSRTSGSSGQ STDNSLLGYP QQIEPSFEPE DEDSEEDDII IEDNGVPQQI PKAVPNTESL ESLTQIELSF ESGQNGFIPS NEVSLAAIAS SSNTEFETHA PDQKLLEEDG TIITL.
Product Science Overview
Structure and Domains
PARD6B contains several important domains:
- PDZ Domain: This domain is involved in protein-protein interactions and is crucial for the formation of multi-protein complexes.
- OPR Domain: The OPR (Oligomerization of Par6) domain is essential for the oligomerization of Par6 proteins.
- CRIB Domain: The semi-Cdc42/Rac interactive binding (CRIB) domain allows PARD6B to interact with small GTPases like Cdc42 and Rac .
Function
PARD6B is a cytoplasmic protein that is involved in various cellular processes:
- Asymmetrical Cell Division: PARD6B plays a role in the asymmetric division of cells, which is essential for the development of multicellular organisms.
- Cell Polarization: It is involved in establishing cell polarity, which is crucial for the function and organization of tissues.
- Centrosome Organization: PARD6B is essential for the organization and function of the centrosome, a key cellular structure involved in microtubule organization .
Role in Disease
PARD6B has been implicated in various diseases, particularly in cancer. Dysregulation of PARD6B can lead to abnormal cell division and migration, contributing to cancer progression and metastasis. For example, studies have shown that PARD6B is involved in the epithelial-mesenchymal transition (EMT) process, which is a key step in cancer metastasis .
Recombinant PARD6B
Recombinant PARD6B is produced using recombinant DNA technology, which involves inserting the gene encoding PARD6B into a suitable expression system, such as bacteria or yeast, to produce the protein in large quantities. This recombinant protein is used in various research applications to study its function and role in disease .
Applications in Research
Recombinant PARD6B is used in a variety of research applications, including:
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