PAK4 Human

p21 Activated Kinase 4 Human Recombinant

Cat. No.

BT12088

Source

Escherichia Coli.

Synonyms

PAK-4, p21 (CDKN1A)-Activated Kinase 4, p21-Activated Kinase 4, Serine/threonine-protein kinase PAK 4, KIAA1142, PAK4.

Appearance

Sterile filtered colorless solution.

Purity

Greater than 90% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

PAK4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 628 amino acids (1-591 a.a.) and having a molecular mass of 68.3 kDa.
PAK4 is fused to 37 amino acid His-Tag at N-terminus and is purified by standard chromatography techniques.

Product Specs

Introduction

PAK4, a member of the group B p21-activated kinases (PAKs), acts as an effector protein for cell division cycle 42 (Cdc42) and plays a crucial role in cytoskeletal organization and cell morphology regulation. Highly expressed in numerous cancer cell lines, PAK4 is implicated in tumorigenesis. PAK proteins, as significant effectors, link Rho GTPases to cytoskeleton rearrangement and nuclear signaling. These proteins, targets for the small GTP binding proteins Cdc42 and Rac, are involved in a wide array of biological activities. Specifically interacting with the GTP-bound form of Cdc42Hs, PAK4 weakly activates the JNK family of MAP kinases. It mediates filopodia formation and participates in actin cytoskeleton reorganization. Additionally, PAK4 activates the JNK pathway and phosphorylates/inactivates the protein phosphatase SSH1, resulting in increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. This decreased cofilin activity may contribute to actin filament stabilization.

Description

PAK4 Human Recombinant, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 628 amino acids (1-591 a.a.) with a molecular weight of 68.3 kDa. This protein is fused to a 37 amino acid His-Tag at the N-terminus and purified using standard chromatography techniques.

Physical Appearance

A clear, colorless solution that has been sterilized by filtration.

Formulation

The PAK4 protein solution (0.5mg/ml) is formulated in a buffer containing 25mM HEPES (pH 7.5), 150mM NaCl, 5mM MgCl2, and 30% glycerol.

Stability

For short-term storage (up to 4 weeks), store the entire vial at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.

Purity

The purity of PAK4 Human Recombinant is greater than 90% as determined by SDS-PAGE analysis.

Synonyms

PAK-4, p21 (CDKN1A)-Activated Kinase 4, p21-Activated Kinase 4, Serine/threonine-protein kinase PAK 4, KIAA1142, PAK4.

Source

Escherichia Coli.

Amino Acid Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMFG KRKKRVEISA PSNFEHRVHT GFDQHEQKFT GLPRQWQSLI EESARRPKPL VDPACITSIQ PGAPKTIVRG SKGAKDGALT LLLDEFENMS VTRSNSLRRD SPPPPARARQ ENGMPEEPAT TARGGPGKAG SRGRFAGHSE AGGGSGDRRR AGPEKRPKSS REGSGGPQES SRDKRPLSGP DVGTPQPAGL ASGAKLAAGR PFNTYPRADT DHPSRGAQGE PHDVAPNGPS AGGLAIPQSS SSSSRPPTRA RGAPSPGVLG PHASEPQLAP PACTPAAPAV PGPPGPRSPQ REPQRVSHEQ FRAALQLVVD PGDPRSYLDN FIKIGEGSTG IVCIATVRSS GKLVAVKKMD LRKQQRRELL FNEVVIMRDY QHENVVEMYN
SYLVGDELWV VMEFLEGGAL TDIVTHTRMN EEQIAAVCLA VLQALSVLHA QGVIHRDIKS DSILLTHDGR VKLSDFGFCA QVSKEVPRRK SLVGTPYWMA PELISRLPYG PEVDIWSLGI MVIEMVDGEP PYFNEPPLKA MKMIRDNLPP RLKNLHKVSP SLKGFLDRLL VRDPAQRATA AELLKHPFLA KAGPPASIVP LMRQNRTR.

Product Science Overview

Introduction

p21-Activated Kinase 4 (PAK4) is a member of the p21-activated kinases (PAKs) family, which are serine/threonine kinases. PAK4 is known for its role as an effector protein for cell division cycle 42 (Cdc42) and is involved in various cellular processes, including cytoskeletal organization, cell morphology, and tumorigenesis.

Structure and Function

PAK4 is part of the group B family of PAKs, which also includes PAK5 and PAK6. These kinases are characterized by their ability to bind to and be activated by small GTPases such as Cdc42 and Rac1. Upon activation, PAK4 undergoes autophosphorylation, which enhances its kinase activity and allows it to phosphorylate various downstream targets.

Role in Cancer

PAK4 is highly expressed and hyperactivated in many cancer types, including breast, gastric, prostate, and pancreatic cancers. It plays a central role in the reorganization of the cytoskeleton, which is crucial for cancer cell migration and invasion. Overexpression of PAK4 has been associated with poor prognosis in non-small cell lung cancer (NSCLC) and promotes migration and invasion of cancer cells.

Mechanisms of Action

PAK4 is involved in several intracellular signaling pathways. It acts as a key downstream effector of the K-Ras pathway and the Rho family of GTPases (Rho, Rac, and Cdc42). PAK4 regulates cytoskeleton reorganization, cell proliferation, survival, and motility. It also plays a role in gene transcription pathways by modulating nucleo-cytoplasmic trafficking of β-catenin.

Therapeutic Potential

Given its significant role in cancer progression, PAK4 is considered a potential therapeutic target. Inhibiting PAK4 activity could potentially reduce cancer cell proliferation, migration, and invasion. Research is ongoing to develop specific inhibitors that can target PAK4 and its associated signaling pathways.

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PAK4 Human

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