PABPN1 Human

Poly(A) Binding Protein, Nuclear 1 Human Recombinant

Cat. No.

BT4410

Source

Escherichia Coli.

Synonyms

Poly(A) Binding Protein Nuclear 1, Poly(A) Binding Protein 2, Polyadenylate-Binding Nuclear Protein 1, PABP-2, OPMD, PAB2, Nuclear Poly(A)-Binding Protein 1, PABII.

Appearance

Sterile Filtered clear solution.

Purity

Greater than 95% as determined by SDS-PAGE.

Usage

THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

PABPN1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 211 amino acids (119-306) and having a molecular mass of 23.8 kDa. PABPN1 is fused to a 23 amino acid His-tag at N-terminus.

Product Specs

Introduction

Poly(A) binding protein nuclear 1 (PABPN1) is a highly abundant protein found within the nucleus, known for its strong affinity to newly synthesized poly(A) tails on RNA molecules. PABPN1 is crucial for the proper and efficient extension of these poly(A) tails located at the 3' ends of messenger RNA (mRNA) transcripts in eukaryotic cells. It plays a key role in regulating the length of these tails, maintaining them at approximately 250 nucleotides. While PABPN1 is typically confined to the nucleus, other types of poly(A) binding proteins exist and are found in the cytoplasm. PABPN1 itself is characterized by a repeating sequence of three nucleotides, GCG, at the beginning of its coding region. An abnormal expansion of this repeat, from the usual 6 copies to a range of 8-13 copies, is linked to a genetic disorder known as oculopharyngeal muscular dystrophy (OPMD). Interestingly, there are inactive copies of the PABPN1 gene (pseudogenes) found on chromosomes 19 and X. Furthermore, there's an unusual occurrence where the process of transcription continues beyond the end of the PABPN1 gene and into the neighboring gene, BCL2L2 (BCL2-like 2), a phenomenon termed 'read-through transcription'.

Description

Recombinant human PABPN1, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 211 amino acids (residues 119-306), with a molecular weight of 23.8 kDa. This PABPN1 protein includes a 23-amino acid His-tag fused at its N-terminus.

Physical Appearance

A clear solution that has been sterilized by filtration.

Formulation

The provided solution of PABPN1 (at a concentration of 1mg/ml) contains 20mM Tris-HCl buffer (with a pH of 8.0), 0.4M Urea, and 10% glycerol.

Stability

For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing should be avoided.

Purity

Purity exceeding 95%, as determined by SDS-PAGE analysis.

Synonyms

Poly(A) Binding Protein Nuclear 1, Poly(A) Binding Protein 2, Polyadenylate-Binding Nuclear Protein 1, PABP-2, OPMD, PAB2, Nuclear Poly(A)-Binding Protein 1, PABII.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSLEAIKAR VREMEEEAEK LKELQNEVEK QMNMSPPPGN AGPVIMSIEE KMEADARSIY VGNVDYGATA EELEAHFHGC GSVNRVTILC DKFSGHPKGF AYIEFSDKES VRTSLALDES LFRGRQIKVI PKRTNRPGIS TTDRGFPRAR YRARTTNYNS SRSRFYSGFN SRPRGRVYRG RARATSWYSP Y

Product Science Overview

Structure and Expression

PABPN1 is a single, non-glycosylated polypeptide chain consisting of 211 amino acids (119-306) with a molecular mass of approximately 23.8 kDa. The recombinant form of PABPN1 is often produced in Escherichia coli (E. coli) and includes a 23 amino acid His-tag at the N-terminus to facilitate purification.

Function and Mechanism

PABPN1 enhances the processivity of poly(A) polymerase, which is responsible for adding the poly(A) tail to the 3’ end of mRNA molecules. This polyadenylation process is critical for mRNA stability, nuclear export, and translation efficiency. PABPN1 binds to the poly(A) tail and interacts with other proteins to regulate the length of the poly(A) tail, ensuring proper mRNA maturation and function.

Cellular Localization and Dynamics

PABPN1 shuttles between the nucleus and the cytoplasm. It accumulates in the nucleus upon transcription inhibition, suggesting that active transcription is required for its export. The nuclear import of PABPN1 is an energy-dependent process, and its intracellular distribution can vary based on expression levels. In low-expressing cells, PABPN1 is primarily localized in the cytoplasm, while in high-expressing cells, it accumulates in the nucleus.

Role in Disease

Mutations in the PABPN1 gene are associated with oculopharyngeal muscular dystrophy (OPMD), a late-onset genetic disorder characterized by progressive muscle weakness. These mutations lead to the formation of intranuclear inclusions and disrupt normal PABPN1 function, affecting mRNA processing and stability.

Applications in Research

Recombinant PABPN1 is widely used in research to study mRNA polyadenylation, RNA-protein interactions, and the molecular mechanisms underlying diseases like OPMD. Its ability to bind poly(A) RNA with high affinity makes it a valuable tool for investigating the regulation of gene expression at the post-transcriptional level.

Storage and Stability

For optimal stability, recombinant PABPN1 should be stored at -20°C for long-term storage, with the addition of a carrier protein such as 0.1% HSA or BSA to prevent degradation. It is recommended to avoid multiple freeze-thaw cycles to maintain protein integrity.

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PABPN1 Human

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