p85a Bovine

Phosphoinositide 3-kinase a, regulatory subunit Bovine Recombinant
Cat. No.
BT1682
Source
Leishmania tarentolae.
Synonyms
Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase p85 subunit alpha, PtdIns-3-kinase p85-alpha, PI3K, P85a.
Appearance
Sterile filtered liquid formualtion.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Phosphoinositide 3-kinase subunit p85a Bovine Recombinant has a molecular weight of 83.5 kDa.

Product Specs

Introduction
The p85α subunit functions as the regulatory subunit of class IA PI3-kinase isoforms α, β, and δ. It possesses two SH2 domains, enabling it to bind to tyrosine-phosphorylated growth factor receptors or substrate adaptor proteins. Additionally, p85α contains a BH (breakpoint cluster region homology) domain that exhibits GAP activity towards small GTPases such as Rab4, Rab5, Cdc42, and Rac1, with weaker activity towards Rab6 and Rab11. Notably, PI3Kα-mediated phosphorylation of p85α has been shown to diminish the lipid kinase activity of the heterodimer, suggesting PI3K-dependent signaling events that may not necessitate the production of 3'-phosphorylated phosphoinositides. The protein kinase activity of PI3Kα has been implicated in IRS-1 serine phosphorylation in insulin-stimulated adipocytes and in the phosphorylation of both STAT3 and IRS-1 upon activation of the type 1 IFN receptor by IFN-α.
Description
Recombinant Bovine Phosphoinositide 3-kinase subunit p85α has a molecular weight of 83.5 kDa.
Physical Appearance
Sterile filtered liquid solution.
Formulation
Supplied at a concentration of 1 mg/ml in a buffer composed of 25mM HEPES (pH 8.0), 25mM NaCl, 2.5mM MgCl2, and 50% glycerol.
Stability
For optimal storage, the product should be kept at 4°C if the entire vial will be used within 2-4 weeks. For extended storage, it is recommended to freeze the product at -20°C. To ensure long-term stability during frozen storage, the addition of a carrier protein (0.1% HSA or BSA) is advised. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 90.0% as assessed by SDS-PAGE.
Synonyms
Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase p85 subunit alpha, PtdIns-3-kinase p85-alpha, PI3K, P85a.
Source
Leishmania tarentolae.

Product Science Overview

Introduction

Phosphoinositide 3-kinases (PI3Ks) are a family of enzymes involved in various cellular functions, including cell growth, proliferation, differentiation, motility, survival, and intracellular trafficking . These enzymes play a crucial role in intracellular signaling pathways and are implicated in several diseases, including cancer.

Structure and Function

PI3Ks are heterodimeric enzymes composed of a catalytic subunit (p110) and a regulatory subunit (p85). The regulatory subunit is essential for the stability and proper functioning of the catalytic subunit . The regulatory subunit of PI3K, particularly the p85α isoform, is encoded by the PIK3R1 gene . This subunit contains two SH2 domains, which allow it to bind to activated protein tyrosine kinases, thereby regulating their activity .

Role in Cellular Signaling

PI3Ks are activated by various cell surface receptors, including G protein-coupled receptors and tyrosine kinase receptors . Upon activation, PI3Ks phosphorylate the 3’ position of the inositol ring of phosphatidylinositol (PtdIns), leading to the production of phosphatidylinositol (3,4,5)-trisphosphate (PIP3) . PIP3 serves as a second messenger, recruiting and activating downstream signaling proteins, such as AKT, which are involved in cell survival and growth .

Implications in Disease

The PI3K/AKT signaling pathway is frequently dysregulated in cancer, making it a target for therapeutic intervention . Mutations in the PIK3R1 gene, which encodes the regulatory subunit, have been associated with various cancers . Additionally, the PI3K pathway is involved in insulin signaling and glucose metabolism, implicating it in metabolic disorders such as diabetes .

Bovine Recombinant PI3K

Recombinant proteins are produced through recombinant DNA technology, which involves inserting the gene encoding the protein of interest into a host organism, such as bacteria or yeast, to produce the protein in large quantities. Bovine recombinant PI3K refers to the PI3K protein derived from cattle and produced using recombinant DNA technology. This recombinant protein is used in research to study the structure, function, and regulation of PI3K, as well as its role in various diseases.

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