p59-Fyn Human

p59-Fyn, also known as Proto-oncogene tyrosine-protein kinase Fyn, is a member of the Src-family of kinases. This family of non-receptor protein tyrosine kinases plays a crucial role in various cellular processes, including cell growth, differentiation, and survival . The human recombinant form of p59-Fyn is often used in research to study its functions and interactions.

p59-Fyn is a membrane-associated tyrosine kinase. It has a unique N-terminal domain that interacts with the CD3 and eta chains of the T-cell receptor (TcR). This interaction is essential for T-cell activation and signaling . The protein also contains SH2 and SH3 domains, which allow it to bind to other proteins, such as p82 and p116, acting as substrates or regulators of Fyn activity .

p59-Fyn is highly expressed in the brain, suggesting it plays a significant role in the sensory nervous network and myelination during early stages of central nervous system (CNS) formation . Its expression in the brain indicates its potential involvement in neural development and function.

p59-Fyn associates with the p85 subunit of phosphatidylinositol 3-kinase (PI3K) and interacts with the fyn-binding protein . These interactions are crucial for various signaling pathways that regulate cell growth and survival. Additionally, p59-Fyn has been implicated in the control of cell growth, making it a critical player in oncogenic processes .

The human recombinant form of p59-Fyn is typically expressed in Escherichia coli (E. coli) and purified using proprietary chromatographic techniques . The recombinant protein is often used in various applications, including enzyme-linked immunosorbent assays (ELISA), inhibition assays, and Western blotting .

Researchers use p59-Fyn (Human Recombinant) to study its role in T-cell activation, neural development, and oncogenic processes. Its interactions with other proteins and involvement in critical signaling pathways make it a valuable tool for understanding cellular mechanisms and developing therapeutic strategies.