OXSM Human
3-Oxoacyl-ACP Synthase, Mitochondrial Human Recombinant
Cat. No.
BT24472
Source
Escherichia Coli.
Synonyms
3-Oxoacyl-ACP Synthase Mitochondrial, Type II Mitochondrial Beta-Ketoacyl Synthase, 3-Ketoacyl-Acyl Carrier Protein Synthase, FASN2D, KASI, EC 2.3.1, Beta-Ketoacyl-ACP Synthase.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
OXSM Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 455 amino acids (28-459) and having a molecular mass of 48.1kDa.
OXSM is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
OXSM is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
OXSM, a crucial enzyme for the elongation of fatty acid chains within mitochondria, functions as a beta-ketoacyl synthetase. Research has identified multiple transcript variants of OXSM generated through alternative splicing.
Description
Recombinantly produced in E.coli, OXSM Human is a non-glycosylated polypeptide chain devoid of any post-translational modifications. This protein comprises 455 amino acids, spanning from position 28 to 459, resulting in a molecular weight of 48.1kDa. For purification and ease of use, a 23 amino acid His-tag is fused to the N-terminus of OXSM. The purification process utilizes proprietary chromatographic techniques to ensure high purity.
Physical Appearance
The product is a clear solution that has undergone sterile filtration.
Formulation
The OXSM solution is prepared at a concentration of 0.5mg/ml and is supplied in a buffer containing 20mM Tris-HCl (pH8.0), 0.15M NaCl, 1mM DTT, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the OXSM solution should be stored at 4°C. For extended storage, it is recommended to store the solution in frozen aliquots at -20°C. To further enhance stability during long-term storage, the addition of a carrier protein such as 0.1% HSA or BSA is advised. Repeated freezing and thawing of the solution should be avoided.
Purity
SDS-PAGE analysis confirms that the purity of OXSM Human is greater than 90.0%.
Synonyms
3-Oxoacyl-ACP Synthase Mitochondrial, Type II Mitochondrial Beta-Ketoacyl Synthase, 3-Ketoacyl-Acyl Carrier Protein Synthase, FASN2D, KASI, EC 2.3.1, Beta-Ketoacyl-ACP Synthase.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSKRKFFGT VPISRLHRRV VITGIGLVTP LGVGTHLVWD RLIGGESGIV SLVGEEYKSI PCSVAAYVPR GSDEGQFNEQ NFVSKSDIKS MSSPTIMAIG AAELAMKDSG WHPQSEADQV ATGVAIGMGM IPLEVVSETA LNFQTKGYNK VSPFFVPKIL VNMAAGQVSI RYKLKGPNHA VSTACTTGAH AVGDSFRFIA HGDADVMVAG GTDSCISPLS LAGFSRARAL STNSDPKLAC RPFHPKRDGF VMGEGAAVLV LEEYEHAVQR RARIYAEVLG YGLSGDAGHI TAPDPEGEGA LRCMAAALKD AGVQPEEISY INAHATSTPL GDAAENKAIK HLFKDHAYAL AVSSTKGATG HLLGAAGAVE AAFTTLACYY QKLPPTLNLD CSEPEFDLNY VPLKAQEWKT EKRFIGLTNS FGFGGTNATL CIAGL.
Product Science Overview
Gene and Protein Information
Function and Mechanism
The primary function of OXSM is to catalyze the condensation reaction between acyl carrier protein (ACP)-bound acyl groups and malonyl-ACP, resulting in the formation of 3-oxoacyl-ACP. This reaction is a key step in the elongation cycle of fatty acid biosynthesis . The enzyme’s activity is crucial for the production of longer chain fatty acids, which are necessary for optimal mitochondrial function .
Biological Significance
OXSM is involved in several important biological processes, including:
- Mitochondrial Fatty Acid Synthesis: The enzyme is essential for the synthesis of fatty acids within the mitochondria, which are vital for maintaining mitochondrial membrane integrity and function .
- Lipoic Acid Biosynthesis: OXSM may also play a role in the biosynthesis of lipoic acid, a cofactor required for the proper functioning of several mitochondrial enzyme complexes .
Clinical Relevance
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