Mouse OX40L
Sf9, Baculovirus cells.
Tumor necrosis factor ligand superfamily member 4, OX40 ligand, OX40L, OX-40L, CD252, Tnfsf4, Ox40l, Txgp1l, gp3, OX4, Ath-1, Ath1, CD134L, gp34, Tnlg2b, Txgp1l
Sterile filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
OX40L Mouse produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 157 amino acids (51-198 aa) and having a molecular mass of 17.7kDa.
OX40L is fused to a 6 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
OX40 Ligand, a member of the tumor necrosis factor (TNF) receptor family, plays a crucial role in immune response. Its receptor, OX40, is primarily found on activated CD4+ T cells. OX40 Ligand is expressed on various antigen-presenting cells, including macrophages, DC2s, and activated B lymphocytes. The expression of OX40 is transient and occurs after T cell receptor engagement. The interaction between OX40 and OX40L stimulates the proliferation and differentiation of activated B cells, enhances immunoglobulin secretion, and provides a survival signal for T cells, ultimately contributing to the development of memory T cells.
Recombinant Mouse OX40L, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain. It comprises 157 amino acids (51-198 aa), resulting in a molecular mass of 17.7 kDa. The protein is fused to a 6 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Sterile filtered, colorless solution.
The OX40L solution is provided at a concentration of 1 mg/ml and contains 10% Glycerol and Phosphate-Buffered Saline (pH 7.4).
For short-term storage (2-4 weeks), store the vial at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Greater than 90.0% purity as determined by SDS-PAGE analysis.
Biological activity is determined by its binding ability in a functional ELISA using Human TNFRSF4 (Catalog # cyt-1039).
Tumor necrosis factor ligand superfamily member 4, OX40 ligand, OX40L, OX-40L, CD252, Tnfsf4, Ox40l, Txgp1l, gp3, OX4, Ath-1, Ath1, CD134L, gp34, Tnlg2b, Txgp1l
Sf9, Baculovirus cells.
ADPSSSPAKD PPIQRLRGAV TRCEDGQLFI SSYKNEYQTM EVQNNSVVIK CDGLYIIYLK GSFFQEVKID LHFREDHNPI SIPMLNDGRR IVFTVVASLA FKDKVYLTVN APDTLCEHLQ INDGELIVVQ LTPGYCAPEG SYHSTVNQVP LHHHHHH
Product Science Overview
The murine OX40L cDNA encodes a protein consisting of 198 amino acids, which includes a 28 amino acid N-terminal cytoplasmic domain, a 20 amino acid transmembrane segment, and a 150 amino acid C-terminal extracellular domain . OX40L is primarily expressed on antigen-presenting cells (APCs) such as activated B cells, dendritic cells, and macrophages . It can also be found on activated endothelial cells and CD4+ T cells during antigen stimulation .
OX40L binds to its receptor, OX40 (CD134), which is expressed on activated T cells. This interaction is pivotal for T cell activation and immune response modulation. When OX40 on T cells binds to OX40L on APCs, it leads to enhanced T cell survival, proliferation, and cytotoxicity . Additionally, it promotes the formation of memory T cells and reduces the immunosuppressive functions of regulatory T cells (Tregs) .
Recombinant Mouse OX40 Ligand is produced using a mouse myeloma cell line, NS0-derived mouse OX40L protein, which includes an N-terminal 10-His tag . The recombinant protein is typically purified to a high degree of purity (>95%) and is used in various research applications, including immunology and oncology studies .
OX40L has been extensively studied for its role in immune regulation and its potential as a therapeutic target in cancer immunotherapy. OX40 agonists are being tested in clinical trials, both as monotherapy and in combination with other immunotherapy agents, particularly immune checkpoint inhibitors . The goal is to harness the immune-stimulatory properties of OX40/OX40L interactions to enhance anti-tumor responses .
