OPTC Human

Opticin Human Recombinant
Cat. No.
BT2779
Source
Escherichia Coli.
Synonyms
Opticin, Oculoglycan, OPT.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

OPTC Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 336 amino acids (20-332 a.a) and having a molecular mass of 37.6kDa.
OPTC is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Opticin, also referred to as OPTC, belongs to the class III small leucine-rich repeat protein (SLRP) family. These proteins are typically associated with the extracellular matrix. OPTC is found in significant amounts within the vitreous humor of the eye and is also present in the cornea, iris, ciliary body, optic nerve, choroid, retina, and fetal liver. It is believed that OPTC interacts with collagen fibrils through non-covalent bonds, playing a role in regulating their morphology, spacing, and overall organization. The gene encoding OPTC is situated within a region on chromosome 1 that has been linked to inherited eye disorders, including age-related macular degeneration (AMD) and posterior column ataxia with retinosa pigmentosa (AXPC1).
Description
Recombinant Human OPTC, produced in E. coli, is a single polypeptide chain that lacks glycosylation. It comprises 336 amino acids, spanning from position 20 to 332, and has a molecular weight of 37.6 kDa. This protein includes a 23-amino acid His-tag at its N-terminus to facilitate purification, which is achieved using proprietary chromatographic methods.
Physical Appearance
The solution is sterile, colorless, and has been filtered for clarity.
Formulation
The OPTC protein is supplied in a solution with a concentration of 0.5 mg/ml. The solution is buffered with 20 mM Tris-HCl at a pH of 8.0 and contains 10% glycerol as a stabilizing agent.
Stability
For short-term storage (up to 2-4 weeks), the product should be kept refrigerated at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein such as HSA or BSA (0.1%) is advisable for long-term storage to maintain protein stability. Repeated freezing and thawing of the product should be avoided to prevent degradation.
Purity
The purity of OPTC is determined by SDS-PAGE analysis and is consistently found to be greater than 85%.
Synonyms
Opticin, Oculoglycan, OPT.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSASLPRKE RKRREEQMPR EGDSFEVLPL RNDVLNPDNY GEVIDLSNYE ELTDYGDQLP EVKVTSLAPA TSISPAKSTT APGTPSSNPT MTRPTTAGLL LSSQPNHGLP TCLVCVCLGS SVYCDDIDLE DIPPLPRRTA YLYARFNRIS RIRAEDFKGL TKLKRIDLSN NLISSIDNDA FRLLHALQDL ILPENQLEAL PVLPSGIEFL DVRLNRLQSS GIQPAAFRAM EKLQFLYLSD NLLDSIPGPL PLSLRSVHLQ NNLIETMQRD VFCDPEEHKH TRRQLEDIRL DGNPINLSLF PSAYFCLPRL PIGRFT.

Product Science Overview

Genetic and Molecular Characteristics

Opticin is encoded by the OPTC gene, which is located on chromosome 1q31–q32. This region is also associated with susceptibility to age-related macular degeneration (AMD), a leading cause of vision loss in older adults . The OPTC gene produces a protein that is approximately 62 kDa in size and is characterized by leucine-rich repeats, which are typical of the SLRP family .

Expression and Localization

Opticin is uniquely expressed in various tissues of the eye, including the cornea, iris, ciliary body, vitreous, choroid, and retina . Its expression is not limited to the eye, as it has also been detected in other tissues, albeit at lower levels. The protein’s localization within the eye suggests that it plays a significant role in ocular health and function.

Role in Disease

Research has shown that opticin is involved in the pathobiology of certain diseases. For instance, it has been observed that opticin is uniquely expressed and translocated to the nucleus of chronic lymphocytic leukemia (CLL) cells . This unique expression pattern in CLL cells, but not in healthy control donors, suggests that opticin may have a role in the development or progression of this type of leukemia. Additionally, mutations in the OPTC gene have been investigated in relation to primary open-angle glaucoma (POAG), normal-tension glaucoma (NTG), and AMD .

Research and Therapeutic Potential

The unique expression and localization of opticin in various tissues, particularly in the eye, make it a potential target for therapeutic interventions. Understanding the molecular mechanisms underlying opticin’s function and its role in disease could lead to the development of new treatments for ocular diseases and certain types of leukemia.

In conclusion, opticin (human recombinant) is a significant protein within the SLRP family, with crucial roles in maintaining the structural integrity of the eye and potential implications in disease pathobiology. Further research is needed to fully elucidate its functions and therapeutic potential.

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