OmpA S.Enteritidis

Salmonella Enteritidis Outer Membrane Protein-A Recombinant
Cat. No.
BT9940
Source
Escherichia Coli.
Synonyms
Outer Membrane Protein-A, OmpA.
Appearance
Sterile Filtered clear solution.
Purity
Protein is >95% pure as determined by 10% PAGE (coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

The Recombinant  Salmonella Enteritidis Outer Membrane Protein A, E.Coli derived, 330 amino acids, contains the ompA immunodominant regions. The protein is fused to a His tag at C-terminal and purified by standard chromatography techniques.

Product Specs

Introduction
OmpA protein is a key outer membrane protein found in various Gram-negative bacteria, including A.salmonicida, Shigella dysenteriae, and E.coli. It plays a crucial role in maintaining cell structure, morphology, and porin activity. OmpA also contributes to conjugation and bacteriophage binding. In atypical Aeromonas salmonicida, the causative agent of goldfish ulcer disease, virulence is linked to the production of a paracrystalline outer membrane A-layer protein. This study involved cloning the structural gene responsible for the monomeric form of A-protein from atypical A.salmonicida into a pET-3d plasmid. This enabled the expression and production of a recombinant form of the protein in E.coli BL21(DE3). The induced protein was then isolated from inclusion bodies through a solubilization-renaturation process and further purified using ion exchange chromatography (Q-Sepharose) to achieve over 95% purity. The recombinant A-protein was subsequently compared to A-protein isolated directly from atypical A.salmonicida using biochemical, immunological, and molecular methods. This comparative analysis involved utilizing both glycine and membrane extraction methods for isolating A-protein from the bacterial cells.
Description
This recombinant Salmonella Enteritidis Outer Membrane Protein A is derived from E. coli and comprises 330 amino acids. It encompasses the immunodominant regions of ompA. The protein is engineered with a C-terminal His tag and undergoes purification using standard chromatography methods.
Physical Appearance
The product is a clear solution that has been sterilized through filtration.
Stability
For optimal stability, OmpA S.Enteritidis Recombinant should be stored at -18°C. It can remain stable at 4°C for up to one week. It is important to avoid repeated freeze-thaw cycles.
Formulation
The formulation consists of PBS (Phosphate Buffered Saline) with a concentration of 25mM Arginine.
Purity
Analysis by 10% SDS-PAGE, followed by Coomassie staining, reveals that the protein purity exceeds 95%.
Applications
This product is suitable for use in immunoassays. The outer membrane protein A (ompA) of S. Enteritidis is directly exposed on the bacterium's surface. Research has shown that hens infected with S. Enteritidis, or immunized with ompA DNA vaccines, produce antibodies against ompA. Therefore, vaccinating hens with ompA protein represents a promising approach to reduce S. Enteritidis contamination in eggs, thereby mitigating the risk of human foodborne illness associated with egg consumption.
Synonyms
Outer Membrane Protein-A, OmpA.
Source
Escherichia Coli.

Product Science Overview

Introduction

Salmonella Enteritidis is a serovar of Salmonella enterica, a Gram-negative bacterium belonging to the Enterobacteriaceae family. It is a significant pathogen responsible for foodborne illnesses in humans. The outer membrane proteins (OMPs) of Salmonella play crucial roles in its pathogenicity, including adhesion, invasion, and evasion of the host immune system. One such protein is the Outer Membrane Protein-A (OmpA), which has been studied for its potential as a vaccine candidate and its role in immune response modulation.

Structure and Function of OmpA

OmpA is a well-conserved protein found in the outer membrane of Gram-negative bacteria. It consists of an N-terminal β-barrel domain that spans the outer membrane and a C-terminal periplasmic domain. The β-barrel domain forms a pore that allows the passage of small molecules, while the periplasmic domain interacts with peptidoglycan, providing structural stability to the bacterial cell wall.

In Salmonella Enteritidis, OmpA is involved in various functions:

  • Adhesion and Invasion: OmpA facilitates the adhesion of Salmonella to host cells and contributes to the invasion process. It interacts with host cell receptors, promoting bacterial entry into the cells.
  • Immune Evasion: OmpA helps Salmonella evade the host immune system by inhibiting the complement system, a part of the immune response that targets pathogens for destruction.
  • Structural Integrity: OmpA maintains the structural integrity of the bacterial cell wall, which is essential for the survival of the bacterium in hostile environments.
Recombinant OmpA

Recombinant OmpA refers to the protein produced through recombinant DNA technology. This involves cloning the gene encoding OmpA into an expression vector, which is then introduced into a host organism, such as Escherichia coli, to produce the protein in large quantities. The recombinant OmpA can be purified and used for various applications, including vaccine development and immunological studies.

Applications of Recombinant OmpA
  • Vaccine Development: Due to its role in immune response modulation, recombinant OmpA is being explored as a potential vaccine candidate. Studies have shown that immunization with recombinant OmpA can induce a protective immune response against Salmonella infections .
  • Diagnostic Tools: Recombinant OmpA can be used in the development of diagnostic assays to detect Salmonella infections. The protein can serve as an antigen in serological tests to identify antibodies against Salmonella in patient samples.
  • Research: Recombinant OmpA is used in research to study the mechanisms of Salmonella pathogenicity and host-pathogen interactions. It provides insights into how Salmonella adheres to and invades host cells, as well as how it evades the immune system.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.