OLA1 Human

Obg-Like ATPase 1 Human Recombinant

Cat. No.

BT26044

Source

Escherichia Coli.

Synonyms

Obg-like ATPase 1, DNA damage-regulated overexpressed in cancer 45, DOC45, GTP-binding protein 9, OLA1, GTPBP9, PRO2455, PTD004, GBP45, GTBP9.

Appearance

Sterile Filtered clear solution.

Purity

Greater than 95% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

OLA1 Human Recombinant produced in E. coli is a single polypeptide chain containing 420 amino acids (1-396) and having a molecular mass of 47.3kDa.
OLA1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

Obg-like ATPase 1 (OLA1) is a protein that has been identified as a negative regulator of the cellular response to oxidative stress. This regulatory function is independent of transcriptional processes. Instead of affecting gene expression, OLA1 appears to curb the antioxidant response through non-transcriptional mechanisms. OLA1 possesses the ability to hydrolyze ATP, and to a lesser extent, GTP. Notably, OLA1 levels are significantly reduced in response to agents that induce DNA damage. While OLA1 is found in all tissues, it is particularly abundant in the testis, liver, lung, and brain. Interestingly, elevated levels of OLA1 have been observed in several types of cancer, including cancers of the colon, rectum, ovary, lung, stomach, and uterus.

Description

This product consists of the recombinant human OLA1 protein, which has been produced in E. coli. The protein is a single polypeptide chain comprised of 420 amino acids (specifically, amino acids 1 through 396) and has a molecular weight of 47.3 kDa. For purification and detection purposes, the OLA1 protein has been engineered with a 24-amino acid His-tag at its N-terminus. Purification is achieved using proprietary chromatographic methods.

Physical Appearance

A clear solution that has been sterilized through filtration.

Formulation

The OLA1 protein is provided at a concentration of 1 mg/ml in a solution containing 20 mM Tris-HCl buffer at a pH of 8.0, 10% glycerol, and 0.1 M NaCl.

Stability

For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure the protein's stability during long-term storage, adding a carrier protein (either HSA or BSA) to a final concentration of 0.1% is advised. Repeated freezing and thawing of the product should be avoided.

Purity

The purity of this product is greater than 95%, as determined by SDS-PAGE analysis.

Synonyms

Obg-like ATPase 1, DNA damage-regulated overexpressed in cancer 45, DOC45, GTP-binding protein 9, OLA1, GTPBP9, PRO2455, PTD004, GBP45, GTBP9.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMPPKKG GDGIKPPPII GRFGTSLKIG IVGLPNVGKS TFFNVLTNSQ ASAENFPFCT IDPNESRVPV PDERFDFLCQ YHKPASKIPA FLNVVDIAGL VKGAHNGQGL GNAFLSHISA CDGIFHLTRA FEDDDITHVE GSVDPIRDIE IIHEELQLKD EEMIGPIIDK LEKVAVRGGD KKLKPEYDIM CKVKSWVIDQ KKPVRFYHDW NDKEIEVLNK HLFLTSKPMV YLVNLSEKDY IRKKNKWLIK IKEWVDKYDP GALVIPFSGA LELKLQELSA EERQKYLEAN MTQSALPKII KAGFAALQLE YFFTAGPDEV RAWTIRKGTK APQAAGKIHT DFEKGFIMAE VMKYEDFKEE GSENAVKAAG KYRQQGRNYI VEDGDIIFFK FNTPQQPKKK.

Product Science Overview

Structure and Function

OLA1 is a 45 kDa protein that is widely present in the cytoplasm and expressed in most tissues. It belongs to the YchF subfamily of the Obg-like GTPase family. Unlike other GTPases, OLA1 has higher binding and hydrolysis efficiencies for ATP than for GTP. This unique characteristic allows it to play a distinct role in cellular metabolism and stress responses.

Interaction and Regulation

The encoded protein interacts with breast cancer-associated gene 1 (BRCA1) and BRCA1-associated RING domain protein (BARD1), which are crucial for DNA repair and centrosome regulation. Overexpression of OLA1 has been observed in multiple types of cancer and is often associated with poor survival outcomes.

Role in Cancer

Research has shown that OLA1 plays a significant role in cancer cell proliferation and metastasis. For instance, in oral squamous cell carcinoma (OSCC), OLA1 has been found to inhibit metastasis through the TGFβ/SMAD2 axis. This suggests that OLA1 could be a potential target for cancer therapy, particularly in cancers where it is overexpressed.

Clinical Implications

Given its involvement in critical cellular processes and its association with cancer, OLA1 is a protein of interest for both basic research and clinical applications. Understanding its function and regulation could lead to new therapeutic strategies for cancer treatment and potentially other diseases where cellular stress responses are disrupted.

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OLA1 Human

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