OGG1 Mouse
HMMH, HOGG1, MUTM, OGH1, AP lyase, OGG1, 8-Oxoguanine DNA Glycosylase, OGG1.
Description
OGG1 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 368 amino acids (1-345 a.a) and having a molecular mass of 41.3kDa. OGG1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
HMMH, HOGG1, MUTM, OGH1, AP lyase, OGG1, 8-Oxoguanine DNA Glycosylase, OGG1.
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Product Science Overview
OGG1 is a bifunctional glycosylase, meaning it has two main activities:
- Glycosylase Activity: It cleaves the glycosidic bond of the mutagenic lesion.
- Lyase Activity: It causes a strand break in the DNA backbone .
In mice, the OGG1 gene is located on chromosome 6 and encodes a protein that is highly conserved across species, including humans . The enzyme has a conserved N-terminal domain that contributes residues to the 8-oxoguanine binding pocket, organized into a single copy of a TBP-like fold .
OGG1 exists in multiple isoforms due to alternative splicing. These isoforms are classified into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 isoforms end with exon 7, while type 2 isoforms end with exon 8. The main form of OGG1 that localizes to the mitochondria is OGG1-2a, whereas OGG1-1a localizes to the nucleus due to a nuclear localization signal at its C-terminal end .
The primary role of OGG1 is to maintain genomic stability by repairing oxidative DNA damage. Mice lacking OGG1 have been found to have a normal lifespan but exhibit a higher probability of developing cancer. Interestingly, disruption of the MTH1 gene in OGG1 knockout mice suppresses lung cancer development, highlighting the complex interplay between different DNA repair pathways .
Recombinant OGG1, such as the mouse recombinant version, is produced using recombinant DNA technology. This involves cloning the OGG1 gene into an expression vector, introducing it into a host cell (e.g., E. coli), and purifying the expressed protein. Recombinant OGG1 is used in various research applications to study DNA repair mechanisms, oxidative stress responses, and the development of therapeutic interventions for diseases associated with oxidative DNA damage.
