OCM Human

The recombinant human Oncomodulin-1 is produced in E. coli and has a molecular mass of approximately 13.21 kDa. It consists of 117 amino acid residues and is fused to a 9 amino acid His tag at the N-terminus . Oncomodulin-1 contains two EF-hand domains, which are helix-loop-helix structural domains capable of binding calcium ions with high affinity .

Oncomodulin-1 serves several important biological functions:

1. Calcium Binding: As a high-affinity calcium ion-binding protein, Oncomodulin-1 plays a crucial role in calcium signaling pathways.
2. Growth Regulation: It exhibits calmodulin-like activity, which is involved in enzyme activation and growth regulation .
3. Axon Regeneration: Oncomodulin-1 acts as a macrophage-derived growth factor that promotes axon regeneration in retinal ganglion cells (RGCs). It is secreted by activated macrophages in the vitreous and retina in response to inflammatory conditions that promote optic nerve regeneration .

Recombinant human Oncomodulin-1 is used in various research applications, including:

• Western Blotting: It is commonly used as a protein marker in Western blotting experiments.
• Cancer Research: Due to its presence in tumors, Oncomodulin-1 is studied for its potential role in cancer development and progression .
• Neuroscience Research: Its ability to promote axon regeneration makes it a valuable tool in neuroscience research, particularly in studies related to optic nerve injuries .

The recombinant human Oncomodulin-1 is typically lyophilized and stored at -20°C. After reconstitution, it should be aliquoted to avoid repeated freezing and thawing cycles. The reconstituted protein can be stored at 4°C for a limited period without significant changes in its properties .