MNKEILAVVE AVSNEKALPR EKIFEALESA LATATKKKYE QEIDVRVQID RKSGDFDTFRRWLVVDEVTQ PTKEITLEAA RYEDESLNLG DYVEDQIESV TFDRITTQTA KQVIVQKVREAERAMVVDQF REHEGEIITG VVKKVNRDNI SLDLGNNAEA VILREDMLPR ENFRPGDRVR GVLYSVRPEA RGAQLFVTRS KPEMLIELFR IEVPEIGEEV IEIKAAARDP GSRAKIAVKT NDKRIDPVGA CVGMRGARVQ AVSTELGGER IDIVLWDDNP AQFVINAMAP ADVASIVVDE DKHTMDIAVE AGNLAQAIGR NGQNVRLASQ LSGWELNVMT DDLQAKHQA EAHAAIDTFT KYLDIDEDFA TVLVEEGFST LEELAYVPMK ELLEIEGLDE PTVEALRERA KNALATIAQA QEESLGDNKP ADDLLNLEGV DRDLAFKLAA RGVCTLEDLA EQGIDDLADI EGLTDEKAGA LIMAARNICW FGDEA.
The Transcription Termination/Antitermination L Factor, also known as NusA, is a crucial protein in Escherichia coli (E. coli) that plays a significant role in both transcription termination and antitermination processes. This protein is essential for the regulation of gene expression and the proper functioning of the bacterial transcription machinery.
The NusA gene was first identified through the isolation of the nusAl mutation, which restricts the growth of bacteriophage λ (lambda) by preventing the antitermination activity of the λ N protein . This discovery highlighted the importance of NusA in the transcriptional regulation of both bacterial and phage genes.
NusA is a single, non-glycosylated polypeptide chain consisting of 495 amino acids, with a molecular mass of approximately 54 kDa . The recombinant form of NusA is produced in E. coli and is typically available as a sterile, filtered, colorless solution. It is formulated in a phosphate-buffered saline (PBS) solution with a pH of 7.4 .
NusA is involved in both Rho-dependent and intrinsic transcription termination. In Rho-dependent termination, NusA is essential for the proper functioning of the Rho protein, which terminates transcription by binding to the nascent RNA and causing the RNA polymerase to dissociate from the DNA template . NusA also plays a role in intrinsic termination by inducing conformational changes in the RNA polymerase, preventing RNA interaction with the RpoA subunit, and promoting the formation of hairpin structures at termination sites .
In addition to its role in termination, NusA is also involved in transcriptional antitermination. It aids in the read-through of RNA polymerase genes such as rpoB and rpoC, as well as the successful synthesis of ribosomal RNA genes . NusA interacts with various components of the transcription machinery, including the core RNA polymerase complex and the sigma70 factor, to modulate transcriptional processes .
The recombinant form of NusA is widely used in research to study transcriptional regulation and the mechanisms of termination and antitermination. Understanding the function of NusA and its interactions with other transcription factors is crucial for elucidating the complex regulatory networks that control gene expression in bacteria.