NudC Domain Containing 2 (NUDCD2) is a protein-coding gene that plays a significant role in various cellular processes. This gene is part of the NudC family, which is known for its involvement in protein folding and stabilization. The human recombinant form of NUDCD2 is produced using recombinant DNA technology, which allows for the expression of the protein in host cells such as E. coli.
The NUDCD2 gene is located on chromosome 5 and encodes a protein that consists of 180 amino acids . The protein has a molecular mass of approximately 20 kDa and includes a NudC domain, which is crucial for its function. The recombinant form of NUDCD2 is often fused with a His-tag at the N-terminus to facilitate purification .
NUDCD2 is predicted to enable unfolded protein binding activity and is involved in protein folding . It is primarily located in the cytosol, intercellular bridge, and mitotic spindle . The protein is also associated with the microtubules and cytokinetic bridge, indicating its role in cell division and intracellular transport .
The human recombinant form of NUDCD2 is produced in E. coli as a single, non-glycosylated polypeptide chain . The production process involves the insertion of the NUDCD2 gene into an expression vector, which is then introduced into E. coli cells. The cells express the protein, which is subsequently purified using chromatographic techniques. The His-tag at the N-terminus aids in the purification process, ensuring a high yield of the recombinant protein .
Recombinant NUDCD2 is used in various research applications, including studies on protein folding, cell division, and intracellular transport. Its role in the LIS1/dynein pathway makes it a valuable tool for understanding the molecular mechanisms underlying these processes.