NUBP1 Human

Nucleotide Binding Protein 1 Human Recombinant
Cat. No.
BT1698
Source
Escherichia Coli.
Synonyms
Cytosolic Fe-S cluster assembly factor NUBP1 isoform 1 , NBP, NBP1, NBP35, Nucleotide-binding protein 1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

NUBP1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 343 amino acids (1-320 a.a) and having a molecular mass of 36.9kDa.
NUBP1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques

Product Specs

Introduction
Cytosolic Fe-S cluster assembly factor NUBP1 isoform 1, also known as NUBP1, plays a crucial role in regulating centrosome duplication similarity. This protein is an essential component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery, which is responsible for the maturation of extra-mitochondrial Fe-S proteins. NUBP1 forms a heterotetramer with NUBP2, creating a Fe-S scaffold complex. This complex facilitates the de novo synthesis of Fe-S clusters and their subsequent transfer to target apoproteins.
Description
This product is a recombinant human NUBP1 protein produced in E. coli. It is a single, non-glycosylated polypeptide chain consisting of 343 amino acids (amino acids 1-320), with a molecular weight of 36.9 kDa. A 23-amino acid His-tag is fused to the N-terminus of the protein to facilitate purification, which is achieved using proprietary chromatographic techniques.
Physical Appearance
The product is a clear solution that has been sterilized by filtration.
Formulation
The NUBP1 protein is supplied in a solution at a concentration of 1 mg/ml. The solution is phosphate-buffered saline (PBS) at pH 7.4 and contains 10% glycerol and 1 mM DTT.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure optimal stability during long-term storage, adding a carrier protein such as HSA or BSA at a concentration of 0.1% is advisable. Avoid repeated freeze-thaw cycles to maintain product integrity.
Purity
The purity of the NUBP1 protein is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
Cytosolic Fe-S cluster assembly factor NUBP1 isoform 1 , NBP, NBP1, NBP35, Nucleotide-binding protein 1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMEEVPHD CPGADSAQAG RGASCQGCPN QRLCASGAGA TPDTAIEEIK EKMKTVKHKI LVLSGKGGVG KSTFSAHLAH GLAEDENTQI ALLDIDICGP SIPKIMGLEG EQVHQSGSGW SPVYVEDNLG VMSVGFLLSS PDDAVIWRGP KKNGMIKQFL RDVDWGEVDY LIVDTPPGTS DEHLSVVRYL ATAHIDGAVI ITTPQEVSLQ DVRKEINFCR KVKLPIIGVV ENMSGFICPK CKKESQIFPP TTGGAELMCQ DLEVPLLGRV PLDPLIGKNC DKGQSFFIDA PDSPATLAYR SIIQRIQEFC NLHQSKEENL ISS.

Product Science Overview

Structure and Function

NUBP1 is characterized by its ability to bind and hydrolyze ATP, which is critical for its function in cellular processes . The protein is involved in the assembly of Fe-S clusters, which are important cofactors for many enzymes involved in electron transport, DNA repair, and other metabolic processes . The Fe-S clusters are assembled in the mitochondria and then transported to other parts of the cell where they are incorporated into various proteins .

Genetic and Molecular Insights

The gene encoding NUBP1 is located on chromosome 16 in humans . The protein is highly conserved across different species, indicating its essential role in cellular functions . Mutations or dysregulation of NUBP1 can lead to various cellular dysfunctions and have been implicated in certain diseases .

Recombinant NUBP1

Recombinant NUBP1 is produced using recombinant DNA technology, which involves inserting the gene encoding NUBP1 into a suitable expression system, such as bacteria or yeast, to produce the protein in large quantities . This recombinant protein is used in various research applications to study its function and role in cellular processes .

Research and Applications

Research on NUBP1 has provided significant insights into its role in cellular metabolism and its potential implications in diseases . Studies have shown that NUBP1 is involved in the regulation of mitochondrial function and cellular iron homeostasis . Additionally, NUBP1 has been studied for its potential role in cancer and other diseases where cellular metabolism is disrupted .

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