NTMT1 Human

N-Terminal Xaa-Pro-Lys N-Methyltransferase 1 Human Recombinant
Cat. No.
BT11698
Source
Escherichia Coli.
Synonyms
NTMT1, N-Terminal Xaa-Pro-Lys N-Methyltransferase 1, X-Pro-Lys N-Terminal Protein Methyltransferase 1A, Alpha N-Terminal Protein Methyltransferase 1A , Methyltransferase-Like Protein 11A , N-Terminal RCC1 Methyltransferase, METTL11A, C9orf32, NTM1A, NRMT, Chromosome 9 Open Reading Frame 32, Methyltransferase Like 11A, EC 2.1.1.244, AD-003, HOMT1A, NRMT1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

NTMT1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 248 amino acids (1-223 a.a) and having a molecular mass of 28.1kDa. NTMT1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
N-terminal Xaa-Pro-Lys N-methyltransferase 1, also known as NTMT1, is a member of the methyltransferase superfamily. This enzyme facilitates the transfer of a methyl group from S-adenosyl-l-methionine to the protein ?-amine. This process results in the formation of S-adenosyl-l-homocysteine and ?-N-methylated proteins. NTMT1 is considered a promising target for anticancer therapies due to its overexpression in gastrointestinal cancers and its critical role in cell mitosis.
Description
Recombinant human NTMT1, expressed in E. coli, is a single, non-glycosylated polypeptide chain. This protein consists of 248 amino acids (with a sequence spanning from amino acid positions 1 to 223) and possesses a molecular weight of 28.1 kDa. For purification purposes, a 25 amino acid His-tag is fused to the N-terminus of the NTMT1 protein. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The NTMT1 protein solution is provided at a concentration of 0.5 mg/ml. The solution is buffered with 20 mM Tris-HCl at a pH of 8.0 and also contains 0.15 M NaCl and 10% glycerol.
Stability
For optimal storage, the NTMT1 protein should be kept refrigerated at 4°C if the entire vial will be used within a 2 to 4 week period. For extended storage exceeding this timeframe, it is recommended to freeze the protein solution at -20°C. The addition of a carrier protein such as 0.1% HSA or BSA is advisable for long-term storage. Repeated freezing and thawing of the protein solution should be avoided.
Purity
The purity of NTMT1 protein is determined to be greater than 95% through SDS-PAGE analysis.
Synonyms
NTMT1, N-Terminal Xaa-Pro-Lys N-Methyltransferase 1, X-Pro-Lys N-Terminal Protein Methyltransferase 1A, Alpha N-Terminal Protein Methyltransferase 1A , Methyltransferase-Like Protein 11A , N-Terminal RCC1 Methyltransferase, METTL11A, C9orf32, NTM1A, NRMT, Chromosome 9 Open Reading Frame 32, Methyltransferase Like 11A, EC 2.1.1.244, AD-003, HOMT1A, NRMT1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSEFMTSEV IEDEKQFYSK AKTYWKQIPP TVDGMLGGYG HISSIDINSS RKFLQRFLRE GPNKTGTSCA LDCGAGIGRI TKRLLLPLFR EVDMVDITED FLVQAKTYLG EEGKRVRNYF CCGLQDFTPE PDSYDVIWIQ WVIGHLTDQH LAEFLRRCKG
SLRPNGIIVI KDNMAQEGVI LDDVDSSVCR DLDVVRRIIC SAGLSLLAEE RQENLPDEIY HVYSFALR.

Product Science Overview

Introduction

N-Terminal Xaa-Pro-Lys N-Methyltransferase 1 (NTMT1), also known as METTL11A, is a protein-coding gene that encodes an enzyme responsible for the methylation of the N-terminus of target proteins. This enzyme plays a crucial role in various cellular processes, including DNA damage response and mitosis. The human recombinant form of NTMT1 is produced through recombinant DNA technology, allowing for its use in research and therapeutic applications.

Gene and Protein Structure

The NTMT1 gene is located on chromosome 9 and is also referred to by several aliases, including C9orf32 and HOMT1A . The protein encoded by this gene is a distributive alpha-N-methyltransferase that specifically methylates the N-terminus of proteins containing the N-terminal motif [Ala/Gly/Pro/Ser]-Pro-Lys when the initiator methionine is cleaved . This enzyme can catalyze mono-, di-, or tri-methylation of the exposed alpha-amino group of the Ala, Gly, or Ser residue in the [Ala/Gly/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif .

Function and Mechanism

NTMT1 is responsible for the N-terminal methylation of several key proteins, including KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A, and SET . This methylation is essential for normal cellular functions, such as the formation of the bipolar spindle and chromosome segregation during mitosis . NTMT1 also plays a role in the DNA damage response pathway, contributing to the maintenance of genomic stability .

The enzyme’s activity is characterized by its ability to catalyze the trimethylation of the N-terminal glycine in CENPA after the removal of the initiator methionine . Some substrates may be primed by NTMT2-mediated monomethylation, which further highlights the interplay between different methyltransferases in regulating protein function .

Biological Significance

The methylation of N-terminal residues by NTMT1 is a critical post-translational modification that influences protein stability, localization, and interaction with other cellular components. This modification can affect various biological processes, including cell cycle regulation, signal transduction, and gene expression . Dysregulation of NTMT1 activity has been implicated in several diseases, including cancer, where abnormal methylation patterns can lead to altered cellular behavior and tumor progression .

Research and Therapeutic Applications

The human recombinant form of NTMT1 is widely used in research to study its enzymatic activity, substrate specificity, and role in cellular processes. Recombinant NTMT1 is produced using recombinant DNA technology, which involves the insertion of the NTMT1 gene into an expression vector, followed by the expression and purification of the protein in a suitable host system . This allows researchers to obtain large quantities of the enzyme for biochemical and structural studies.

In addition to its research applications, NTMT1 has potential therapeutic implications. Targeting NTMT1 and its associated pathways could provide new strategies for the treatment of diseases characterized by aberrant methylation patterns, such as cancer . The development of specific inhibitors or modulators of NTMT1 activity could offer novel therapeutic approaches for these conditions.

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