NTAL Human

Non-T-cell Activation Linker Human Recombinant
Cat. No.
BT1469
Source
Escherichia Coli.
Synonyms
LAT2, Linker for Activation of T CellsFamily Member 2, HSPC046, LAB, WBSCR15, WBSCR5, WSCR5, Non-T-cell activation linker, Linker for activation of B-cells, Membrane-associated adapter molecule, Williams-Beuren syndrome chromosomal region 5 protein, Williams-Beuren syndrome chromosomal region 15 protein.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 80% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

NTAL Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 238 amino acids (27-243 a.a.) and having a molecular mass of 26.2 kDa. NTAL protein is fused to a 21 amino acid His tag at N-terminus and is purified by standard chromatography.

Product Specs

Introduction
NTAL is an adaptor protein involved in signal transduction pathways initiated by various immune receptors. It plays a crucial role in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells, contributing to allergic responses. Additionally, NTAL participates in BCR (B-cell antigen receptor)-mediated signaling in B cells, essential for antibody production, and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-mediated signaling in myeloid cells, involved in immune complex clearance. NTAL functions by linking the activation of these receptors and their associated kinases to downstream intracellular events through the recruitment of GRB2.
Description
Recombinant human NTAL protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 238 amino acids (residues 27-243). The protein has a molecular weight of 26.2 kDa and includes a 21 amino acid His-tag fused at the N-terminus to facilitate purification by standard chromatography techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The provided solution of recombinant human NTAL is supplied in a buffer consisting of 20mM Tris-HCl at pH 8.0 and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the NTAL solution can be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. To further enhance stability during long-term storage, consider adding a carrier protein such as 0.1% HSA or BSA. Repeated freeze-thaw cycles should be avoided to maintain protein integrity.
Purity
The purity of the recombinant human NTAL protein is greater than 80%, as determined by SDS-PAGE analysis.
Synonyms
LAT2, Linker for Activation of T CellsFamily Member 2, HSPC046, LAB, WBSCR15, WBSCR5, WSCR5, Non-T-cell activation linker, Linker for activation of B-cells, Membrane-associated adapter molecule, Williams-Beuren syndrome chromosomal region 5 protein, Williams-Beuren syndrome chromosomal region 15 protein.
Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MRCSRPGAKR SEKIYQQRSL REDQQSFTGS RTYSLVGQAW PGPLADMAPT RKDKLLQFYP SLEDPASSRY QNFSKGSRHG SEEAYIDPIA MEYYNWGRFS KPPEDDDANS YENVLICKQK TTETGAQQEG IGGLCRGDLS LSLALKTGPT SGLCPSASPE EDEESEDYQN SASIHQWRES RKVMGQLQRE ASPGPVGSPD EEDGEPDYVN GEVAATEA.

Product Science Overview

Introduction

Non-T-cell Activation Linker (NTAL), also known as Linker for Activation of B cells (LAB), is a transmembrane adaptor protein. It is structurally and evolutionarily related to the Linker for Activation of T cells (LAT). NTAL is expressed in various immune cells, including B cells, natural killer (NK) cells, mast cells, and macrophages .

Structure and Expression

NTAL is a 30 kDa double-palmitoylated protein. It is rapidly phosphorylated after the engagement of B cell receptors (BCR) or Fc receptors (FcR) . The protein is encoded by the WBSCR5 gene, which is located in the Williams-Beuren syndrome chromosomal region . The amino acid sequence of NTAL includes several tyrosine residues that become phosphorylated upon activation, facilitating the recruitment of downstream signaling molecules .

Function

NTAL plays a crucial role in the negative regulation of early stages of BCR-dependent B cell signaling. It serves as a negative regulator in mast cells as well, although it also contributes to some activation processes in these cells, partially overlapping with LAT function . In T cells, NTAL negatively regulates T cell receptor (TCR) signaling, which is essential to avoid uncontrolled immune responses .

Research and Applications

Recent studies have shown that NTAL expression in Jurkat cells, a model for T cells, decreases calcium fluxes and PLC-γ1 activation upon stimulation through the TCR complex . This suggests that NTAL has a potential role in autoimmune disorders, such as rheumatoid arthritis, by negatively regulating TCR signaling .

Recombinant NTAL proteins are used in various research applications, including blocking assays and control experiments . These proteins are typically expressed in systems like E. coli and purified for use in laboratory settings .

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