NSL1 Human

NSL1 contains two coiled-coil domains and is localized to kinetochores, which are chromosome-associated structures that attach to microtubules and mediate chromosome movements throughout cell division . The recombinant human NSL1 protein is typically expressed in Escherichia coli and is a single, non-glycosylated polypeptide chain containing 304 amino acids (1-281) with a molecular mass of approximately 34.6 kDa .

NSL1 plays a pivotal role in the MIS12 complex, which is a conserved protein complex that includes two chromodomain-containing proteins and a component of the outer plate of the kinetochore . This complex is responsible for connecting centromeric heterochromatin with the outer kinetochore structure, ensuring accurate chromosome segregation during cell division .

Recombinant human NSL1 protein is widely used in research, particularly in studies related to cell division and chromosome dynamics. It is often utilized in SDS-PAGE and other biochemical assays to understand its role and interactions within the MIS12 complex .

The recombinant NSL1 protein is typically stored at 4°C if used within 2-4 weeks, or at -20°C for longer periods. It is recommended to add a carrier protein (0.1% HSA or BSA) for long-term storage to avoid multiple freeze-thaw cycles .