NPPC Human

Natriuretic Peptide C Human Recombinant
Cat. No.
BT19462
Source
Escherichia Coli.
Synonyms
Natriuretic Peptide Precursor C, C-Type Natriuretic Peptide, CNP2, CNP.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

NPPC Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 126 amino acids (24-126) and having a molecular mass of 13.2kDa.
NPPC is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Natriuretic peptide precursor C (NPPC) undergoes proteolytic processing to yield a secreted hormone belonging to the natriuretic peptide family. NPPC exhibits vasoactive and natriuretic properties, playing a role in regulating blood pressure and fluid balance. Moreover, it influences the growth and differentiation of chondrocytes within the cartilaginous growth plate, contributing to skeletal development.
Description
Recombinant human NPPC, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 126 amino acids (residues 24-126) with a molecular weight of 13.2 kDa. This protein encompasses amino acids 24 to 126 of the NPPC sequence and incorporates a 23-amino acid His-tag at the N-terminus to facilitate purification using proprietary chromatographic techniques.
Physical Appearance
Clear solution subjected to sterile filtration.
Formulation
The NPPC solution is provided at a concentration of 0.25 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the NPPC solution should be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. To enhance stability during long-term storage, the addition of a carrier protein such as 0.1% HSA or BSA is advised. It is important to minimize repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of the NPPC protein is determined to be greater than 85% as assessed by SDS-PAGE analysis.
Synonyms
Natriuretic Peptide Precursor C, C-Type Natriuretic Peptide, CNP2, CNP.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSKPGAPPK VPRTPPAEEL AEPQAAGGGQ KKGDKAPGGG GANLKGDRSR LLRDLRVDTK SRAAWARLLQ EHPNARKYKG ANKKGLSKGC FGLKLDRIGS MSGLGC

Product Science Overview

Introduction

Natriuretic Peptide C (CNP) is a member of the natriuretic peptide family, which also includes Atrial Natriuretic Peptide (ANP) and Brain Natriuretic Peptide (BNP). These peptides play crucial roles in cardiovascular homeostasis by regulating blood pressure, blood volume, and sodium balance. CNP is encoded by the NPPC gene in humans .

Structure and Function

CNP is synthesized as a precursor protein, natriuretic peptide precursor C (NPPC), which is then cleaved to produce the active 22-amino acid peptide . Unlike ANP and BNP, which are primarily involved in cardiovascular regulation, CNP has a broader range of functions, including roles in bone growth, cartilage development, and reproductive processes .

Mechanism of Action

CNP exerts its effects by binding to natriuretic peptide receptors, specifically NPR-B, which is a receptor guanylyl cyclase. This binding leads to the production of cyclic guanosine monophosphate (cGMP), a secondary messenger that mediates various physiological effects . CNP is known for its vasodilatory, anti-proliferative, and anti-fibrotic properties, making it a potential therapeutic target for various cardiovascular and fibrotic diseases .

Clinical Applications

Recombinant human CNP has been explored for its therapeutic potential in treating conditions such as achondroplasia, a form of dwarfism caused by mutations in the FGFR3 gene. Clinical trials have shown that CNP can promote bone growth and improve skeletal abnormalities in affected individuals . Additionally, CNP’s cardiovascular benefits are being investigated for potential use in treating hypertension and heart failure .

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THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
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