Introduction
Nucleolar protein 3 (NOL3) is a protein found primarily in muscle cells. It is located in the nucleoplasm and is particularly concentrated in the nucleoli, structures within the nucleus. NOL3 acts as an anti-apoptotic protein, meaning it helps prevent cell death. It does this by suppressing the activity of enzymes like caspase 2, caspase 8, and the tumor suppressor protein p53. High levels of NOL3 are found in heart and skeletal muscle, while lower levels are present in organs like the placenta, liver, kidney, and pancreas. Interestingly, NOL3 levels increase with neuronal activity, suggesting a role in neuroplasticity, the brain's ability to change and adapt. NOL3 appears to interact with the structural framework of neuronal cells (the cytoskeleton), particularly with a component called F-Actin. This interaction may be indirect, potentially involving another protein that associates with F-Actin.
Description
This product is a recombinant human NOL3 protein produced in E. coli bacteria. It consists of a single chain of 231 amino acids (specifically, amino acids 1 to 208 of the full NOL3 protein) and has a molecular weight of 25.0 kDa. The protein includes a 23 amino acid His-tag attached to its N-terminus, which aids in purification. The protein has been purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless liquid that has been sterilized by filtration.
Formulation
The NOL3 protein is provided in a solution containing 1mg of protein per 1ml of buffer. The buffer composition is: 20mM Tris-HCl (pH 8.0), 100mM NaCl, 1mM DTT, 1mM EDTA, and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), keep the NOL3 vial refrigerated at 4°C. For long-term storage, freeze the protein at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage. Avoid repeated freezing and thawing of the protein.
Purity
The purity of the NOL3 protein is greater than 90%, as assessed by SDS-PAGE analysis.
Synonyms
Nucleolar protein 3 (apoptosis repressor with CARD domain), Muscle-enriched cytoplasmic protein, Nucleolar protein of 30 kDa, Apoptosis repressor with CARD, nucleolar protein 3, NOP30, CARD2, ARC, NOP, MYP, MYC.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMGNAQER PSETIDRERK RLVETLQADS GLLLDALLAR GVLTGPEYEA LDALPDAERR VRRLLLLVQG KGEAACQELL RCAQRTAGAP DPAWDWQHVG PGYRDRSYDP PCPGHWTPEA PGSGTTCPGL PRASDPDEAG GPEGSEAVQS GTPEEPEPEL EAEASKEAEP EPEPEPELEP EAEAEPEPEL EPEPDPEPEP DFEERDESED S.