NOB1 Human

NIN1/RPN12 Binding Protein 1 Human Recombinant
Cat. No.
BT429
Source
Escherichia Coli.
Synonyms
NIN1/RPN12 Binding Protein 1 Homolog, PSMD8BP1, PSMD8 Binding Protein 1, Nin One Binding Protein, Phosphorylation Regulatory Protein HP-10, Protein ART-4, RNA-Binding Protein NOB1, Adenocarcinoma Antigen Recognized By T Lymphocytes 4, NOB1P, MST158.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

NOB1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 435 amino acids (1-412) and having a molecular mass of 49.1kDa.
NOB1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Nob1 is a protein involved in the final stages of ribosome maturation. It specifically participates in the processing of precursor ribosomal RNA (pre-rRNA) and is responsible for cleaving a 20S rRNA intermediate at the D site. This cleavage event generates the mature 18S rRNA, a crucial component of the small ribosomal subunit. Ribosome assembly in yeast requires over 200 protein and RNA cofactors, many of which are conserved in eukaryotes. These factors regulate the modification and cleavage of the initial 35S pre-rRNA transcript into the mature 18S, 5.8S, and 25S rRNAs. They also facilitate rRNA folding and the incorporation of ribosomal proteins and 5S RNA.
Description
Recombinant human NOB1, expressed in E. coli, is available as a single, non-glycosylated polypeptide chain. The protein consists of 435 amino acids (residues 1-412) and has a molecular weight of 49.1 kDa. For purification purposes, a 23 amino acid His-tag is fused to the N-terminus of NOB1. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
The product is a clear solution that has been sterilized by filtration.
Formulation
NOB1 is supplied in a solution at a concentration of 1 mg/ml. The solution contains 20 mM Tris-HCl buffer (pH 8.0), 0.4 M Urea, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the product frozen at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advisable for extended storage. Avoid repeated freeze-thaw cycles to maintain product integrity.
Purity
The purity of NOB1, as determined by SDS-PAGE analysis, is greater than 80.0%.
Synonyms
NIN1/RPN12 Binding Protein 1 Homolog, PSMD8BP1, PSMD8 Binding Protein 1, Nin One Binding Protein, Phosphorylation Regulatory Protein HP-10, Protein ART-4, RNA-Binding Protein NOB1, Adenocarcinoma Antigen Recognized By T Lymphocytes 4, NOB1P, MST158.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAPVEHV VADAGAFLRH AALQDIGKNI YTIREVVTEI RDKATRRRLA VLPYELRFKE PLPEYVRLVT EFSKKTGDYP SLSATDIQVL ALTYQLEAEF VGVSHLKQEP QKVKVSSSIQ HPETPLHISG FHLPYKPKPP QETEKGHSAC EPENLEFSSF MFWRNPLPNI DHELQELLID RGEDVPSEEE EEEENGFEDR KDDSDDDGGG WITPSNIKQI QQELEQCDVP EDVRVGCLTT DFAMQNVLLQ MGLHVLAVNG MLIREARSYI LRCHGCFKTT SDMSRVFCSH CGNKTLKKVS VTVSDDGTLH MHFSRNPKVL NPRGLRYSLP TPKGGKYAIN PHLTEDQRFP QLRLSQKARQ KTNVFAPDYI AGVSPFVEND ISSRSATLQV RDSTLGAGRR RLNPNASRKK FVKKR

Product Science Overview

Gene and Protein Structure

The NOB1 gene is located on chromosome 16q22.1 and encodes a protein consisting of 412 amino acids . The protein contains a highly conserved PIN domain, which is involved in RNA degradation, and a zinc ribbon domain with four conserved cysteines . These structural features are essential for its function in RNA metabolism and stability .

Function and Mechanism

NOB1 is involved in the processing of pre-ribosomal RNA (pre-rRNA) and the biogenesis of 40S ribosomal subunits . In yeast, NOB1 cleaves the 20S pre-rRNA at cleavage site D to produce mature 18S rRNA . This function is conserved in humans, where NOB1 interacts with pre-40S ribosomal particles and is required for the cytoplasmic conversion of 20S pre-rRNA to mature 18S rRNA .

Biological Significance

NOB1 is ubiquitously expressed in normal tissues such as the lung, liver, and spleen . Its core physiological function is to regulate protease activities and participate in maintaining RNA metabolism and stability . Given its essential role in ribosome assembly, NOB1 is critical for cellular protein synthesis and overall cellular function.

Clinical Relevance

Mutations or dysregulation of NOB1 have been associated with various diseases, including Hermansky-Pudlak Syndrome 2 . Additionally, NOB1 has been identified as a potential biomarker or target in cancer research due to its involvement in RNA processing and ribosome biogenesis .

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