NMRAL1 Human

NmrA-Like Family Domain Containing 1 Human Recombinant
Cat. No.
BT390
Source
E.coli.
Synonyms
NmrA-like family domain containing protein 1, short chain dehydrogenase/reductase family 48A member 1, HSCARG, SDR48A1, FLJ25918.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

NMRAL1 Human Recombinant produced in E. coli is a single polypeptide chain containing 323 amino acids (1-299) and having a molecular mass of 35.9 kDa.
NMRAL1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
NMRAL1, a redox sensor protein, undergoes structural changes and subcellular redistribution in response to fluctuations in intracellular NADPH/NADP+ ratios. Under low NADPH conditions, NMRAL1 primarily exists as a monomer and binds to argininosuccinate synthase (ASS1), an enzyme involved in nitric oxide (NO) production. This interaction inhibits NMRAL1's activity and reduces NO synthesis, consequently suppressing apoptosis. Conversely, when NADPH levels are normal, NMRAL1 predominantly forms dimers, concealing the ASS1 binding site.
Description
Recombinant human NMRAL1, expressed in E. coli, is a single polypeptide chain comprising 323 amino acids (residues 1-299) with a molecular weight of 35.9 kDa. It features an N-terminal 24-amino acid His-tag and is purified using proprietary chromatographic methods.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The NMRAL1 solution is provided at a concentration of 0.5 mg/ml in 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 95% as determined by SDS-PAGE analysis.
Synonyms
NmrA-like family domain containing protein 1, short chain dehydrogenase/reductase family 48A member 1, HSCARG, SDR48A1, FLJ25918.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMVDKKL VVVFGGTGAQ GGSVARTLLE DGTFKVRVVT RNPRKKAAKE LRLQGAEVVQ GDQDDQVIME LALNGAYATF IVTNYWESCS QEQEVKQGKL LADLARRLGL HYVVYSGLEN IKKLTAGRLA AAHFDGKGEV EEYFRDIGVP MTSVRLPCYF ENLLSHFLPQ KAPDGKSYLL SLPTGDVPMD GMSVSDLGPV VLSLLKMPEK YVGQNIGLST CRHTAEEYAA LLTKHTRKVV HDAKMTPEDY EKLGFPGARD LANMFRFYAL RPDRDIELTL RLNPKALTLD QWLEQHKGDF NLL

Product Science Overview

Introduction

NmrA-Like Family Domain Containing 1 (NMRAL1), also known as NmrA-Like Redox Sensor 1, is a protein encoded by the NMRAL1 gene in humans. This protein plays a crucial role in cellular redox homeostasis and is involved in various biological processes, including the regulation of nitric oxide synthesis and the cellular antiviral response.

Gene and Protein Structure

The NMRAL1 gene is located on chromosome 16 and encodes a protein that consists of 299 amino acids . The protein has a molecular weight of approximately 35.9 kDa . It contains a redox-sensing domain that allows it to respond to changes in intracellular NADPH/NADP+ levels .

Biological Functions

NMRAL1 functions as a redox sensor protein. It undergoes structural changes and subcellular redistribution in response to fluctuations in intracellular NADPH/NADP+ levels . At low NADPH concentrations, NMRAL1 exists primarily as a monomer and binds to argininosuccinate synthase (ASS1), an enzyme involved in nitric oxide synthesis . This binding impairs ASS1 activity, reducing nitric oxide production and preventing apoptosis . Under normal NADPH concentrations, NMRAL1 forms a stable dimer, which hides the binding site for ASS1 .

Expression Patterns and Tissue Distribution

NMRAL1 is expressed in various tissues, with higher expression levels observed in the liver, kidney, and heart . Its expression is regulated by cellular redox status, and it plays a key role in maintaining redox homeostasis in these tissues .

Regulatory Mechanisms

NMRAL1 negatively regulates the activity of NF-kappaB in a ubiquitylation-dependent manner . It also plays a critical role in the cellular antiviral response by negatively regulating the interferon response factor 3-mediated expression of interferon-beta . Alternative splicing of the NMRAL1 gene results in multiple transcript variants, which may have distinct regulatory functions .

Recombinant NMRAL1

Recombinant human NMRAL1 protein is produced using E. coli expression systems and is often tagged with a His-tag for purification purposes . This recombinant protein is used in various research applications to study its structure, function, and interactions with other proteins .

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